Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, 41080 Sevilla, Spain.
Hans-Knöll-Institut für Naturstoff-Forschung e. V., D-07745 Jena, Germany.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2515-2519. doi: 10.1099/ijs.0.026195-0. Epub 2010 Nov 26.
A Gram-reaction-positive, motile, coccus-shaped actinobacterium, designated strain T2A-S27(T), was isolated from a roof tile in Oporto (Portugal) and studied using a polyphasic approach. The 16S rRNA gene sequence of the novel isolate showed high similarity to that of Kineococcus marinus KST3-3(T) (97.8 % sequence similarity). Strain T2A-S27(T) showed lower 16S rRNA gene sequence similarities with other members of the genus Kineococcus and members of the family Kineosporiaceae (<94 %). A phylogenetic tree, based on 16S rRNA gene sequences, showed that strain T2A-S27(T) formed a coherent clade with the type strain of K. marinus and Quadrisphaera granulorum. The isolate was characterized by the presence of meso-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H(2)) as the predominant menaquinone and a polar lipid profile consisting of diphosphatidylglycerol and phosphatidylglycerol. The fatty acid profile was dominated by anteiso-C(15 : 0). The DNA G+C content was 76.9 mol%. The low level of DNA-DNA relatedness to K. marinus (46-47 %) and the results of the chemotaxonomic and physiological studies clearly distinguished strain T2A-S27(T) from recognized species of the genus Kineococcus. On the basis of its phylogenetic position and phenotypic traits, strain T2A-S27(T) ( = LMG 24148(T) = CECT 7306(T) = DSM 23768(T)) represents a novel species of a new genus in the family Kineosporiaceae, for which the name Pseudokineococcus lusitanus gen. nov., sp. nov. is proposed. The misclassified species K. marinus is transferred to the new genus as Pseudokineococcus marinus comb. nov. The type strain of Pseudokineococcus marinus is KST3-3(T) ( = KCCM 42250(T) = NRRL B-24439(T)).
一株革兰氏阳性、运动、球菌状放线菌，被命名为 T2A-S27(T) 菌株，从葡萄牙波尔图的一个屋顶瓦片中分离得到，并通过多相研究进行了研究。新型分离株的 16S rRNA 基因序列与海洋动球菌 KST3-3(T)（97.8%序列相似性）高度相似。T2A-S27(T) 菌株与其他金球菌属成员和金孢子科成员的 16S rRNA 基因序列相似性较低，<94%。基于 16S rRNA 基因序列的系统发育树显示，T2A-S27(T) 菌株与海洋动球菌和四方球菌形成一个连贯的分支。该分离株的特征是细胞壁肽聚糖中存在 meso-二氨基庚二酸，主要的menaquinone 为 MK-9(H2)，极性脂谱由双磷脂酰甘油和磷脂酰甘油组成。脂肪酸谱以 anteiso-C(15：0)为主。DNA G+C 含量为 76.9 mol%。与海洋动球菌的 DNA-DNA 同源性低（46-47%），以及化学分类和生理研究的结果，清楚地区分了 T2A-S27(T) 菌株与金球菌属的公认种。基于其系统发育位置和表型特征，T2A-S27(T) 菌株（=LMG 24148(T)=CECT 7306(T)=DSM 23768(T)）代表金孢子科一个新属的新种，建议将其命名为假动球菌属 lusitanus 属，新种。被错误分类的海洋动球菌种被转移到新属，命名为假动球菌属 marinus 种。假动球菌属 marinus 的模式株是 KST3-3(T)（=KCCM 42250(T)=NRRL B-24439(T)）。
