MTCC - Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Sector 39A, Chandigarh, India.
DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2419-2424. doi: 10.1099/ijs.0.025593-0. Epub 2010 Nov 12.
During the course of a study on the bacterial diversity in Western Ghats, India, an actinobacterial strain, designated PC IW02(T), was isolated and characterized by a polyphasic taxonomic approach. Strain PC IW02(T) was a non-motile, Gram-positive, short rod that formed creamish white to yellow coloured colonies. 16S rRNA gene sequence analysis showed that the novel strain showed highest sequence similarity with type strains of members of the genus Dermacoccus: Dermacoccus barathri (96.6 %), Dermacoccus profundi (96.5 %), Dermacoccus abyssi (96.4 %) and Dermacoccus nishinomiyaensis (95.9 %). The phylogenetic tree suggested that strain PC IW02(T) could represent a member of a new genus of the family Dermacoccaceae with the genus Demetria as closest clade. Pairwise sequence alignment with Demetria terragena HKI 0089(T) and Kytococcus sedentarius DSM 20547(T) showed similarities of 94.2 and 93.7 %, respectively. Strain PC IW02(T) had MK-8(H(4)) as the major menaquinone. The major fatty acids were iso-C(16 : 0) (43.4 %), iso-C(16 : 1) H (17.2 %) and anteiso-C(17 : 0) (9.9 %). The diagnostic cell-wall amino acid at position 3 of the peptide subunit was lysine; the interpeptide bridge consisted of Gly-Ser-Asp. The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and phosphatidylserine, along with two unknown phospholipids. The genomic DNA G+C content of the isolate was 77 mol%. On the basis of phenotypic characteristics, including chemotaxonomic data, and 16S rRNA gene sequence similarities, strain PC IW02(T) represents a novel species in a new genus of the family Dermacoccaceae for which the name Calidifontibacter indicus gen. nov., sp. nov. is proposed. The type strain of Calidifontibacter indicus is PC IW02(T) ( = MTCC 8338(T) = DSM 22967(T) = JCM 16038(T)). An emended description of the family Dermacoccaceae is provided.
在对印度西高止山脉细菌多样性的研究过程中，分离并通过多相分类学方法对一株放线菌菌株进行了特征描述，命名为 PC IW02(T)。菌株 PC IW02(T) 是一个非运动的革兰氏阳性短杆菌，形成奶油色至黄色的菌落。16S rRNA 基因序列分析表明，该新菌株与 Dermacoccus 属的模式菌株具有最高的序列相似性：Dermacoccus barathri（96.6%）、Dermacoccus profundi（96.5%）、Dermacoccus abyssi（96.4%）和 Dermacoccus nishinomiyaensis（95.9%）。系统发育树表明，菌株 PC IW02(T) 可能代表 Dermacoccaceae 科的一个新属的成员，与 Demetria 属最为接近。与 Demetria terragena HKI 0089(T) 和 Kytococcus sedentarius DSM 20547(T) 的序列比对显示相似度分别为 94.2%和 93.7%。菌株 PC IW02(T) 的主要生育醌为 MK-8(H(4))。主要脂肪酸为 iso-C(16：0)（43.4%）、iso-C(16：1) H（17.2%）和 anteiso-C(17：0)（9.9%）。肽亚基位置 3 的特征细胞壁氨基酸为赖氨酸；肽桥由 Gly-Ser-Asp 组成。存在的极性脂有二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和磷脂酰丝氨酸，以及两种未知的磷脂。该分离株的基因组 DNA G+C 含量为 77 mol%。基于表型特征，包括化学生物分类数据和 16S rRNA 基因序列相似性，菌株 PC IW02(T) 代表 Dermacoccaceae 科的一个新属的新种，提议命名为 Calidifontibacter indicus gen. nov.，sp. nov.。Calidifontibacter indicus 的模式菌株为 PC IW02(T)（=MTCC 8338(T)=DSM 22967(T)=JCM 16038(T)）。提供了 Dermacoccaceae 科的修订描述。
