Yusubalieva G M, Baklaushev V P, Gurina O I, Tsitrin E B, Chekhonin V P
Department of Fundamental and Applied Neurobiology, V. P. Serbskii National Research Centre for Social and Forensic Psychiatry, Federal Agency for Health Care and Social Development, Moscow, Russia.
Bull Exp Biol Med. 2010 Jul;149(1):125-30. doi: 10.1007/s10517-010-0890-7.
In experiments on Wistar rats with experimental C6 glioma, the immunohistochemical features of the astroglial reaction over 30 days after implantation were characterized. The formation of a glial border consisting of GFAP-positive reactive astrocytes at the periphery of C6 glioma was observed on postimplantation day 3 and until the death of the experimental animals. Reactive astrocytes encompassed not only the primary gliomal focus, but all tumor invasion foci. Quantitative assessment of astroglial reaction around glioma was carried out with immunofluorescent assay of glial fibrillary acidic protein (GFAP) on cerebral sections. The size of glioma and necrotic foci were analyzed morphometrically in parallel with enzyme immunoassay of serum GFAP. A correlation between morphometric indices of glioma and serum level of GFAP was found. It was concluded that serum concentration of GFAP correlated with the size of intracranial glioma, necrotic foci, and most strongly with the degree of reactive astrogliosis. Monitoring of the level of serum GFAP can serve as an additional diagnostic index reporting the state of intracranial glioma.
在对患有实验性C6胶质瘤的Wistar大鼠进行的实验中，对植入后30天内星形胶质细胞反应的免疫组织化学特征进行了表征。在植入后第3天直至实验动物死亡，观察到在C6胶质瘤周边形成了由GFAP阳性反应性星形胶质细胞组成的胶质边界。反应性星形胶质细胞不仅包围了原发性胶质瘤灶，还包围了所有肿瘤侵袭灶。通过对脑切片上的胶质纤维酸性蛋白（GFAP）进行免疫荧光测定，对胶质瘤周围的星形胶质细胞反应进行了定量评估。与血清GFAP的酶免疫测定同时，对胶质瘤和坏死灶的大小进行了形态计量学分析。发现胶质瘤的形态计量学指标与血清GFAP水平之间存在相关性。得出的结论是，GFAP的血清浓度与颅内胶质瘤的大小、坏死灶相关，与反应性星形胶质细胞增生程度的相关性最强。监测血清GFAP水平可作为报告颅内胶质瘤状态的一项额外诊断指标。
