Iacono R F, Nessi de Aviñón A, Rosetti F A, Berría M I
Departamento de Microbiología, Universidad de Buenos Aires, Paraguay, Argentina.
Neurosci Lett. 1995 Nov 24;200(3):175-8. doi: 10.1016/0304-3940(95)12105-d.
Cultured astrocytes derived from newborn rat brain were inoculated with Junin virus (JV) to characterize their response to infection by means of their glial fibrillary acidic protein (GFAP) immunochemical profile. Samples from 1 to 11 days post-inoculation (pi), as well as matched controls, were serially harvested for GFAP labeling by peroxidase-antiperoxidase (PAP) method. It was only at day 3 that significantly greater values of GFAP staining (P < 0.05) were disclosed by three complementary approaches: image analysis, ELISA and immunoblot densitometry. Since such increase was abolished by Triton X-100 treatment, soluble GFAP fraction appeared responsible for the early though transient enhancement of GFAP immunoreactivity that followed viral inoculation.
将新生大鼠脑源性培养星形胶质细胞接种胡宁病毒（JV），通过其胶质纤维酸性蛋白（GFAP）免疫化学特征来表征它们对感染的反应。接种后1至11天（pi）的样本以及配对对照，通过过氧化物酶 - 抗过氧化物酶（PAP）方法连续收集用于GFAP标记。仅在第3天，通过三种互补方法（图像分析、ELISA和免疫印迹密度测定）发现GFAP染色值显著更高（P < 0.05）。由于Triton X-100处理消除了这种增加，可溶性GFAP组分似乎是病毒接种后GFAP免疫反应性早期但短暂增强的原因。
