Max Planck Institute for Colloids and Interfaces, Department of Biomolecular Systems, Research Campus Golm, 14476 Potsdam, Germany.
Nano Lett. 2011 Jan 12;11(1):73-8. doi: 10.1021/nl102821f. Epub 2010 Nov 29.
Biocompatible glyco-dendronized poly-l-lysine (PLL) polymers carry either three or nine mannose- or galactose-bearing dendrons that selectively bind, and thus can be used to detect, bacteria. Central to the synthesis of glyco-dendronized polymers was the development of a continuous flow [2 + 2] photocycloaddition reaction to connect the dendrons and PLL. Glycodendronized polymers cluster bacteria by binding to cell-surface carbohydrate receptors and thereby result in an easy read-out using microscopic analyses.
生物相容的糖基化树枝状聚赖氨酸 (PLL) 聚合物携带三个或九个带有甘露糖或半乳糖的树枝状结构，这些树枝状结构选择性地结合，因此可用于检测细菌。糖基化聚合物合成的核心是开发连续流 [2 + 2] 光环加成反应来连接树枝状结构和 PLL。糖基化聚合物通过与细胞表面碳水化合物受体结合来聚集细菌，从而可以使用显微镜分析轻松进行读取。
