DJ-1诱导的PTEN下调在人胶质瘤细胞迁移和侵袭中的作用

Role of DJ-1-induced PTEN down-regulation in migration and invasion of human glioma cells.

作者信息

Fang Mao, Zhong Xue-Yun, Du Bin, Lin Chen-Li, Luo Feng, Tang Li-Juan, Chen Juan

机构信息

Department of Pathology, Medical College, Jinan University, Guangzhou, Guangdong 510632, P. R. China.

出版信息

Chin J Cancer. 2010 Dec;29(12):988-94. doi: 10.5732/cjc.010.10307.

DOI:10.5732/cjc.010.10307

PMID:21114918

Abstract

BACKGROUND AND OBJECTIVE

DJ-1, a suppressor of PTEN, promotes metastasis of different tumors, but its function and mechanisms in glioma metastasis remain unclear. This study aimed to investigate the effect of the DJ-1 protein on the migration and invasion of human glioma cells, and to explore potential mechanisms.

METHODS

The eukaryotic expression vector pEGFP/DJ-1 and small interfering RNA (siRNA) were constructed and transfected into human glioma SWO-38 cells. The expression of DJ-1 and PTEN in SWO-38 cells were detected by Western blot. Cell migration and invasion were detected by transwell assay.

RESULTS

After transfection of pEGFP/DJ-1, the expression of DJ-1 (1.28 ± 0.15 vs. 0.89 ± 0.04, P < 0.05) and focal adhesion kinase (FAK) phosphorylation (0.76 ± 0.12 vs. 0.51 ± 0.04, P < 0.05) were increased, whereas the expression of PTEN (0.74 ± 0.2 vs. 1.04 ± 0.14, P < 0.05) was suppressed. After transfection of DJ-1 siRNA, both DJ-1 (0.33 ± 0.04 vs. 0.88 ± 0.06, P < 0.05) and p-FAK levels (0.33 ± 0.01 vs. 0.44 ± 0.05, P < 0.05) were decreased, but PTEN expression (1.1 ± 0.06 vs. 0.81 ± 0.12, P < 0.05) was increased. Transwell assay data showed that pEGFP/DJ-1 transfection promoted SWO-38 cell migration (57.2 ± 6.50 vs. 40.4 ± 5.0, P < 0.05) and invasion (54.6 ± 4.9 vs. 27 ± 6.7, P < 0.05), whereas DJ-1 siRNA transfection inhibited SWO-38 cells migration (54.4 ± 6.9 vs. 73.4 ± 7.6, < 0.05) and invasion (44.6 ± 5.8 vs. 69.2 ± 9.2, P < 0.05).

CONCLUSION

Over-expression of DJ-1 promotes SWO-38 cell migration and invasion possibly through the DJ-1 and the PTEN/FAK pathway.

摘要

背景与目的

DJ-1作为PTEN的一种抑制因子，可促进不同肿瘤的转移，但其在胶质瘤转移中的作用及机制尚不清楚。本研究旨在探讨DJ-1蛋白对人胶质瘤细胞迁移和侵袭的影响，并探索其潜在机制。

方法

构建真核表达载体pEGFP/DJ-1和小干扰RNA（siRNA），并将其转染到人胶质瘤SWO-38细胞中。通过蛋白质免疫印迹法检测SWO-38细胞中DJ-1和PTEN的表达。采用Transwell实验检测细胞迁移和侵袭能力。

结果

转染pEGFP/DJ-1后，DJ-1的表达（1.28±0.15 vs. 0.89±0.04，P<0.05）和粘着斑激酶（FAK）的磷酸化水平（0.76±0.12 vs. 0.51±0.04，P<0.05）升高，而PTEN的表达（0.74±0.2 vs. 1.04±0.14，P<0.05）受到抑制。转染DJ-1 siRNA后，DJ-1（0.33±0.04 vs. 0.88±0.06，P<0.05）和p-FAK水平（0.33±0.01 vs. 0.44±0.05，P<0.05）均降低，但PTEN表达（1.1±0.06 vs. 0.81±0.12，P<0.05）升高。Transwell实验数据显示，pEGFP/DJ-1转染促进SWO-38细胞迁移（57.2±6.50 vs. 40.4±5.0，P<0.05）和侵袭（54.6±4.9 vs. 27±6.7，P<0.05），而DJ-1 siRNA转染则抑制SWO-38细胞迁移（54.4±6.9 vs. 73.4±7.6，P<0.05）和侵袭（44.6±5.8 vs. 69.2±9.2，P<0.05）。