Zhu Xiao-Lin, Sun Wei, Lei Wen-Bin, Zhuang Hui-Wen, Hou Wei-Jian, Wen Wei-Ping
Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.
Mol Med Rep. 2015 Aug;12(2):2003-8. doi: 10.3892/mmr.2015.3617. Epub 2015 Apr 15.
DJ-1, a novel mitogen-dependent oncogene, has an important role in the progression of human malignancies, whereas tumor suppressor phosphatase and tensin homolog (PTEN) is known to control a variety of processes associated with cell survival, proliferation and invasion. DJ-1 overexpression was reported to be negatively correlated with PTEN expression in tumor tissues of patients with laryngeal squamous cell carcinoma (LSCC). In the present study, the effect of DJ-1 on PTEN in laryngeal cancer cells was investigated by transfecting DJ-1-specific small interfering (si)RNA into Hep-2 and SNU-899 cells. Cell survival and cell proliferative and invasive capacity were then evaluated. The results showed that siRNA targeting of DJ-1 effectively upregulated PTEN expression, resulting in enhanced cell death as well as decreased proliferation and invasion of Hep-2 and SNU-899 cells. The results of the present study indicated, for the first time, to the best of our knowledge, that DJ-1-induced PTEN downregulation is associated with proliferative and invasive activity of laryngeal cancer cells. The DJ-1 gene may have an important role in the tumorigenesis of LSCC.
DJ-1是一种新型的有丝分裂原依赖性癌基因，在人类恶性肿瘤进展中起重要作用，而肿瘤抑制因子磷酸酶和张力蛋白同源物（PTEN）已知可控制与细胞存活、增殖和侵袭相关的多种过程。据报道，在喉鳞状细胞癌（LSCC）患者的肿瘤组织中，DJ-1过表达与PTEN表达呈负相关。在本研究中，通过将DJ-1特异性小干扰（si）RNA转染到Hep-2和SNU-899细胞中，研究了DJ-1对喉癌细胞中PTEN的影响。然后评估细胞存活以及细胞增殖和侵袭能力。结果表明，靶向DJ-1的siRNA有效上调了PTEN表达，导致Hep-2和SNU-899细胞的细胞死亡增加以及增殖和侵袭减少。据我们所知，本研究结果首次表明，DJ-1诱导的PTEN下调与喉癌细胞的增殖和侵袭活性相关。DJ-1基因可能在LSCC的肿瘤发生中起重要作用。
