College of Animal Sciences, Zhejiang University, Hangzhou, China.
J Insect Physiol. 2011 Feb;57(2):274-9. doi: 10.1016/j.jinsphys.2010.11.015. Epub 2010 Nov 26.
A large volume of honey bee (Apis mellifera) tag-seq was obtained to identify differential gene expression via Solexa/lllumina Digital Gene Expression tag profiling (DGE) based on next generation sequencing. In total, 4,286,250 (foragers) and 3,422,327 (nurses) clean tags were sequenced, 24,568 (foragers) and 13,134 (nurses) distinct clean tags could not be match to the reference database, and 7508 and 6875 mapped genes were detected in foragers and nurses respectively. 7045 genes were found differentially expressed between foragers and nurses. Of those genes, 1621 genes had significantly different expression, that is, they showed an expression ratio (foragers/nurses) of more than 2 and FDR (False Discovery Rate) of less than 0.001. We identified 101 genes that were uniquely expressed in foragers, and 9 genes that were only expressed in nurses. We performed the Gene Ontology (GO) category and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and found 415 genes with annotation terms linked to the GO cellular component category. 200 components of KEGG pathways were obtained, including 21 signaling pathways. The PPAR signaling pathway was the most highly enriched, with the lowest Q-value.
我们获得了大量的蜜蜂（Apis mellifera）标签序列，通过基于下一代测序的 Solexa/lllumina 数字基因表达标签分析（DGE）来鉴定差异基因表达。总共对 4286250 个（采集蜂）和 3422327 个（保育蜂）清洁标签进行了测序，24568 个（采集蜂）和 13134 个（保育蜂）独特的清洁标签无法与参考数据库匹配，在采集蜂和保育蜂中分别检测到 7508 和 6875 个映射基因。在采集蜂和保育蜂之间发现了 7045 个差异表达的基因。在这些基因中，有 1621 个基因的表达差异显著，即它们的表达比（采集蜂/保育蜂）超过 2，FDR（假发现率）小于 0.001。我们鉴定了 101 个在采集蜂中特异性表达的基因，和 9 个仅在保育蜂中表达的基因。我们进行了基因本体论（GO）类别和京都基因与基因组百科全书（KEGG）途径富集分析，发现了 415 个具有与 GO 细胞成分类别相关注释术语的基因。获得了 200 个 KEGG 途径的组成部分，包括 21 个信号通路。PPAR 信号通路是最富集的，Q 值最低。
