Iusupova D V, Bening G P, Sokolova R B, Fedorov R V
Vopr Med Khim. 1990 Jan-Feb;36(1):27-31.
Diphtheritic bacteria of PW-8 Massachusetts strain produced into cultural medium only one nucleotidase--endoDNAase. The enzyme was synthesized by the cells during the exponential phase of growth. The DNAase was purified 500-fold and exhibited properties specific to neutral-alkaline DNAases (pH optimum about 7.5, absolute requirements for Me2+, single-step mechanism of substrate hydrolysis). The following properties were typical for the enzyme: absence of distinct specificity to structure of bases surrounding the hydrolyzed bond, formation of 5'-end phosphate groups and slightly higher preference to denatured DNA.
PW - 8马萨诸塞菌株的白喉杆菌在培养基中仅产生一种核苷酸酶——内切DNA酶。该酶在细胞生长的指数期合成。DNA酶被纯化了500倍，并表现出中性碱性DNA酶的特异性性质（最适pH约为7.5，对Me2 +有绝对需求，底物水解的单步机制）。该酶具有以下典型性质：对水解键周围碱基结构无明显特异性，形成5'-末端磷酸基团，对变性DNA的偏好略高。
