Hayes F, Daly C, Fitzgerald G F
Department of Food Microbiology, University College, Cork, Ireland.
J Bacteriol. 1990 Jun;172(6):3485-9. doi: 10.1128/jb.172.6.3485-3489.1990.
In vivo recombination events involving the 75-kilobase lactose proteinase plasmid pCI301 of Lactococcus lactis subsp. lactis UC317 and the conjugative enterococcal plasmid pAM beta 1 were analyzed. A fragment, identified as containing the pCI301 recombination site, mediated greatly elevated levels of mobilization and recombination with pAM beta 1 when cloned in a nonmobilizable L. lactis-Escherichia coli shuttle vector. This latter recombination event was site and orientation specific on both plasmids. Recombination on pAM beta 1 was within the region associated with plasmid replication, but no effect on pAM beta 1 replication functions was detected. Resolution of recombinant plasmids generated derivatives indistinguishable from the parental plasmids.
对涉及乳酸乳球菌乳酸亚种UC317的75千碱基乳糖蛋白酶质粒pCI301和接合性肠球菌质粒pAMβ1的体内重组事件进行了分析。当克隆到不可移动的乳酸乳球菌-大肠杆菌穿梭载体中时，一个被鉴定为含有pCI301重组位点的片段介导了与pAMβ1的转移和重组水平的大幅提高。后一种重组事件在两种质粒上都是位点和方向特异性的。pAMβ1上的重组发生在与质粒复制相关的区域内，但未检测到对pAMβ1复制功能的影响。重组质粒的拆分产生了与亲本质粒无法区分的衍生物。
