RNA Biology Group and Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
Biochem Soc Trans. 2010 Dec;38(6):1576-80. doi: 10.1042/BST0381576.
Ribosomal progression through the open reading frames within mRNAs is frequently considered as uneventful when compared with the highly regulated initiation step. However, both RNA and nascent peptide can interact with the ribosome to influence how translation proceeds and can modify gene expression in several ways. 2A peptides are a class of sequences that, as nascent chains, pause ribosomes and drive a translation-termination reaction on a sense (proline) codon, followed by continued downstream translation. In the present paper, what is known about the 2A reaction is discussed, and 2A is compared with other sequences that, as nascent peptides, pause or stall translation.
与高度调控的起始步骤相比，核糖体在 mRNA 的开放阅读框中穿过通常被认为是没有事件发生的。然而，RNA 和新生肽都可以与核糖体相互作用，影响翻译的进行，并以几种方式修饰基因表达。2A 肽是一类序列，作为新生链，使核糖体暂停并在一个有意义（脯氨酸）密码子上引发翻译终止反应，然后继续下游翻译。在本文中，讨论了关于 2A 反应的已知信息，并将 2A 与其他作为新生肽暂停或stall 翻译的序列进行了比较。
