Moloney N A, Webbe G
London School of Hygiene and Tropical Medicine.
Parasitology. 1990 Apr;100 Pt 2:235-9. doi: 10.1017/s0031182000061230.
Sera from rabbits, rats and mice multiply-vaccinated with attenuated cercariae of Schistosoma japonicum conferred high levels of resistance against challenge to naive recipient mice (up to 97, 64 and 60% respectively). Vaccinated rabbit and rat sera were given before challenge and vaccinated mouse serum 5 days after challenge. To show that the protective factors in these sera were antibodies, vaccinated rabbit and mouse sera were fractionated by protein A-Sepharose and the fractions precipitated by 50% ammonium sulphate. The protein A-Sepharose binding or non-binding fractions in vaccinated rabbit serum transferred approximately equal levels of significant resistance to mice, suggesting that both the IgG and non-IgG components of vaccinated rabbit serum are protective. The major part of the protective activity in vaccinated mouse serum was transferred to recipients by the protein A-Sepharose binding fraction, i.e. the IgG antibodies. Heat inactivation of sera at 56 degrees C for 3 h affected the protective capacity of vaccinated rat sera, but not that of vaccinated rabbit or mouse sera.
用日本血吸虫减毒尾蚴多次免疫的兔、大鼠和小鼠的血清，能使未接触过抗原的受体小鼠对攻击产生高水平的抵抗力（分别高达97%、64%和60%）。在攻击前给予免疫兔和大鼠的血清，在攻击后5天给予免疫小鼠的血清。为了证明这些血清中的保护因子是抗体，用蛋白A-琼脂糖对免疫兔和小鼠的血清进行分级分离，并用50%硫酸铵沉淀分级分离物。免疫兔血清中与蛋白A-琼脂糖结合或不结合的分级分离物向小鼠传递了大致相同水平的显著抵抗力，这表明免疫兔血清的IgG和非IgG成分都具有保护作用。免疫小鼠血清中的主要保护活性通过蛋白A-琼脂糖结合分级分离物传递给受体，即IgG抗体。血清在56℃加热灭活3小时影响了免疫大鼠血清的保护能力，但不影响免疫兔或小鼠血清的保护能力。
