向大鼠脑黑质注射6-羟基多巴胺：形态学和生物化学效应。

Injections of 6-hydroxydopamine in the substantia nigra of the rat brain: morphological and biochemical effects.

作者信息

Buonamici M, Cervini M A, Rossi A C, Sebastiani L, Raffaelli A, Bagnoli P

机构信息

Farmitalia Carlo Erba, CNS Line, Erbamont Group, Nerviano, Milano, Italy.

出版信息

Behav Brain Res. 1990 Apr 16;38(1):83-95. doi: 10.1016/0166-4328(90)90027-c.

DOI:10.1016/0166-4328(90)90027-c

PMID:2112003

Abstract

Rats with unilateral injections of 6-hydroxydopamine (6-OHDA) into the substantia nigra pars compacta were classified as active and inactive according to the intensity of their spontaneous and/or apomorphine-induced turning behavior (TB), and sacrificed at different survival times for morphological and biochemical analysis. In active rats, at any survival time, dopaminergic fluorescence in the nigrostriatal system as well as dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) content of the nucleus caudatus-putamen drastically decreased on the brain side ipsilateral to the injection. Dopaminergic fluorescence as well as DA and DOPAC content of the mesolimbic system ipsilateral to the injection also decreased. In inactive rats, at any survival time, 6-OHDA-induced lesions only partially involved both nigrostriatal and mesolimbic systems. Our results are indicative of a good correlation between the intensity of TB and the extent of 6-OHDA-induced lesions, as assessed by morphological and biochemical analysis.

摘要

将6-羟基多巴胺（6-OHDA）单侧注射到黑质致密部的大鼠，根据其自发和/或阿扑吗啡诱导的旋转行为（TB）强度分为活跃组和不活跃组，并在不同存活时间处死以进行形态学和生化分析。在活跃组大鼠中，在任何存活时间，注射侧脑同侧黑质纹状体系统中的多巴胺能荧光以及尾状核-壳核中的多巴胺（DA）和3,4-二羟基苯乙酸（DOPAC）含量都急剧下降。注射侧脑同侧中脑边缘系统的多巴胺能荧光以及DA和DOPAC含量也下降。在不活跃组大鼠中，在任何存活时间，6-OHDA诱导的损伤仅部分累及黑质纹状体和中脑边缘系统。我们的结果表明，通过形态学和生化分析评估，TB强度与6-OHDA诱导的损伤程度之间具有良好的相关性。