Moebius U, Manns M, Hess G, Kober G, Meyer zum Büschenfelde K H, Meuer S C
Abteilung Angewandte Immunologie, Deutsches Krebsforschungszentrum, Heidelberg, FRG.
Eur J Immunol. 1990 Apr;20(4):889-96. doi: 10.1002/eji.1830200426.
Immunological events are involved in the pathophysiology of chronic active hepatitis as indicated from the accumulation of T lymphocytes at the site of tissue damage. We generated T cell clones from liver biopsies of 3 patients with chronic active hepatitis B and 2 patients with primary biliary cirrhosis. These T cell clones (n = 84) were analyzed by means of T cell receptor (TcR) beta gene rearrangements to determine whether the infiltrate consists of a polyclonal or oligoclonal T cell population. The vast majority (62 of 64) of T cell clones from three different patients with chronic active hepatitis B showed no identical rearrangements of the TcR beta chain genes. In marked contrast, in both patients with primary biliary cirrhosis, T cell clones established were of limited diversity. Thus 5 out of 10 and 2 out of 10 T cell clones from one patient and 3 out of 9 and 2 out of 9 T cell clones from the second patient, respectively, showed identical TcR beta gene rearrangements. These data suggest that a clonal dominance is characteristic for local T cell responses in autoimmune liver disease such as primary biliary cirrhosis whereas in virus-induced chronic active hepatitis T cell activation occurs polyclonally.
免疫事件参与慢性活动性肝炎的病理生理学过程，这从组织损伤部位T淋巴细胞的聚集可以看出。我们从3例慢性乙型活动性肝炎患者和2例原发性胆汁性肝硬化患者的肝活检中生成T细胞克隆。通过T细胞受体（TcR）β基因重排分析这些T细胞克隆（n = 84），以确定浸润细胞是由多克隆还是寡克隆T细胞群体组成。来自3例不同慢性乙型活动性肝炎患者的绝大多数（64个中的62个）T细胞克隆未显示TcRβ链基因的相同重排。与之形成显著对比的是，在2例原发性胆汁性肝硬化患者中，所建立的T细胞克隆多样性有限。因此，来自一名患者的10个T细胞克隆中有5个以及10个中有2个，分别与来自第二名患者的9个T细胞克隆中的3个以及9个中的2个显示出相同的TcRβ基因重排。这些数据表明，克隆优势是自身免疫性肝病如原发性胆汁性肝硬化中局部T细胞反应的特征，而在病毒诱导的慢性活动性肝炎中，T细胞激活是多克隆发生的。
