具有与 dmp 操纵子同源基因的 Stenotrophomonas 菌株 HPC383 的降解潜力。
Degradative potential of Stenotrophomonas strain HPC383 having genes homologous to dmp operon.
机构信息
Environmental Genomics Unit, National Environmental Engineering Research Institute (NEERI), CSIR, Nehru Marg, Nagpur (MH), India.
出版信息
Bioresour Technol. 2011 Feb;102(3):3227-33. doi: 10.1016/j.biortech.2010.11.016. Epub 2010 Nov 12.
A strain, Stenotrophomonas HPC383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through HPLC and GC analysis. Culture HPC383 could degrade (%) various compounds (1 mM) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% crude oil. Gene locus dmpN, to identify phenol degrading capacity was determined by PCR followed by southern analysis. The sequenced DNA fragment exhibited 99% sequence similarity to phenol hydroxylase gene from Arthrobacter sp. W1 (FJ610336). Amino acid sequence analysis of phenol hydroxylase reveals it to belong to high-Ks (affinity constant) group. Application of HPC383 in bioremediation of aquatic and terrestrial sites contaminated with petrochemical has been suggested.
从处理农药工业废水的污水处理厂中分离出一株 Stenotrophomonas HPC383 菌株，该菌株通过 HPLC 和 GC 分析可降解各种芳香族化合物（甲酚、苯酚、儿茶酚、4-甲基儿茶酚和对苯二酚）和原油。在 48 小时的孵育期内，HPC383 可以降解（%）混合物中的各种化合物（1mM）：苯酚 - 99，对甲酚 - 100，4-甲基儿茶酚 - 96 和对苯二酚 - 43，而降解 0.5% 原油的 94%则需要 7 天时间。通过 PCR 随后进行 Southern 分析确定了识别苯酚降解能力的基因座 dmpN。测序的 DNA 片段与 Arthrobacter sp. W1（FJ610336）中的苯酚羟化酶基因显示出 99%的序列相似性。苯酚羟化酶的氨基酸序列分析表明它属于高-Ks（亲和力常数）组。建议将 HPC383 应用于受石化污染的水和陆地环境的生物修复。
Zotero 插件