Yang Hee-Chan, Im Wan-Taek, Kang Myung Suk, Shin Do-Yun, Lee Sung-Taik
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Int J Syst Evol Microbiol. 2006 Jan;56(Pt 1):81-4. doi: 10.1099/ijs.0.63826-0.
A Gram-negative, rod-shaped, non-spore-forming bacterium (TR6-01T) was isolated from compost near Daejeon city in South Korea. On the basis of 16S rRNA gene sequence similarity, strain TR6-01T was shown to belong to the class Gammaproteobacteria, related to Stenotrophomonas acidaminiphila (97.1 %) and Stenotrophomonas maltophilia (96.9 %); the phylogenetic distance from any other established species within the genus Stenotrophomonas was less than 97.0 %. Phenotypic and chemotaxonomic data (major ubiquinone Q-8; fatty acid profile) supported the affiliation of strain TR6-01T to the genus Stenotrophomonas. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain TR6-01T from the five Stenotrophomonas species with validly published names. TR6-01T therefore represents a novel species, for which the name Stenotrophomonas koreensis sp. nov. is proposed, with the type strain TR6-01T (= KCTC 12211T = JCM 13256T).
从韩国大田市附近的堆肥中分离出一株革兰氏阴性、杆状、不产芽孢的细菌(TR6-01T)。基于16S rRNA基因序列相似性,菌株TR6-01T被证明属于γ-变形菌纲,与嗜氨基寡养单胞菌(97.1%)和嗜麦芽寡养单胞菌(96.9%)相关;与寡养单胞菌属内任何其他已确定的物种的系统发育距离小于97.0%。表型和化学分类数据(主要泛醌Q-8;脂肪酸谱)支持菌株TR6-01T隶属于寡养单胞菌属。DNA-DNA杂交以及生理生化测试结果使得菌株TR6-01T能够与五个已有效发表名称的寡养单胞菌属物种在基因型和表型上得以区分。因此,TR6-01T代表一个新物种,为此提出名称嗜韩国寡养单胞菌(Stenotrophomonas koreensis)新种,模式菌株为TR6-01T(= KCTC 12211T = JCM 13256T)。