Novikov A A, Borukhov S I
Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
Biochem Biophys Res Commun. 1990 May 31;169(1):297-301. doi: 10.1016/0006-291x(90)91467-7.
The plasmid pBINS1, containing the promoter, SD and leader peptide sequences of Bacillus amyloliquefaciens alpha-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of protease-deficient Bacillus subtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.
质粒pBINS1含有解淀粉芽孢杆菌α-淀粉酶基因的启动子、SD序列和前导肽序列以及编码人胰岛素原的267bp长序列，它指导了杂合前胰岛素原的高效合成，以及胰岛素原在蛋白酶缺陷型枯草芽孢杆菌AJ73细胞外的定量分泌。重组胰岛素原已从培养基中分离出来，其N端序列显示与天然人激素原相同。
