Larrodera P, Cornet M E, Diaz-Meco M T, Lopez-Barahona M, Diaz-Laviada I, Guddal P H, Johansen T, Moscat J
Medicina y Cirugía Experimental Hospital General, Gregorio Marañón, Madrid, Spain.
Cell. 1990 Jun 15;61(6):1113-20. doi: 10.1016/0092-8674(90)90074-o.
Recent evidence suggests the involvement of phosphatidylcholine (PC) hydrolysis both in the control of normal cell growth and in transformation. We show here that the simple exogenous addition of Bacillus cereus PC-hydrolyzing phospholipase C (PC-PLC) is sufficient to elicit a potent mitogenic response in Swiss 3T3 fibroblasts by a mechanism that is independent of protein kinase C. Our results on the additivity and synergism between B. cereus PC-PLC, PDGF, and insulin in the mitogenic response indicate that this novel phospholipid degradative pathway may be important in the mitogenic signaling cascade activated by PDGF.
最近的证据表明，磷脂酰胆碱（PC）水解参与正常细胞生长的调控以及细胞转化过程。我们在此表明，简单地外源添加蜡样芽孢杆菌PC水解磷脂酶C（PC-PLC），就足以通过一种独立于蛋白激酶C的机制，在瑞士3T3成纤维细胞中引发强烈的促有丝分裂反应。我们关于蜡样芽孢杆菌PC-PLC、血小板衍生生长因子（PDGF）和胰岛素在促有丝分裂反应中的加和性与协同作用的研究结果表明，这条新的磷脂降解途径可能在由PDGF激活的促有丝分裂信号级联反应中起重要作用。
