Laviada I D, Baudet C, Galve-Roperh I, Naveilhan P, Brachet P
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad Complutense, Madrid, Spain.
FEBS Lett. 1995 May 15;364(3):301-4. doi: 10.1016/0014-5793(95)00414-5.
Addition of phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) to cultured glial cells increased the levels of nerve growth factor (NGF) mRNA and the amount of cell-secreted NGF. The effect of PC-PLC was 2.5 times higher than that elicited by 4 beta-phorbol 12 beta-myristate 13 alpha-acetate. In cells in which protein kinase C (PKC) was fully inhibited or downregulated, the effect of PC-PLC was reduced-though still evident-and similar to that exerted by sphingosine. Results thus indicate that PC-PLC induces the synthesis of NGF by glial cells by a PKC-dependent and PKC-independent mechanisms.
