Department of Orthopaedics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Spine (Phila Pa 1976). 2011 Apr 15;36(8):600-6. doi: 10.1097/BRS.0b013e3181d8bca7.
An experimental study investigating the effect of disc cells on neurite outgrowth in a rat dorsal root ganglion (DRG) culture system.
To examine the effects of the 2 nucleus pulposus (NP) cell populations, notochordal cells (NC) and chondrocyte-like cells (CC) on neurite outgrowth from DRGs.
NP consists of at least 2 cell populations, NC and CC. The cells in NP have been shown to be responsible for negative effects on neurite outgrowth in vitro and on nerve tissue in vivo. It is unknown whether 1 cell type or combinations of the 2 cell types are responsible for the reported effects.
A total of 939 DRGs from newborn Sprague Dawley rats were harvested and placed in culture dishes. After 24 hours, the neurite outgrowth was measured. NP was harvested from tail discs of adult rats and the NP cells were separated into 2 populations, NC and CC. The cell populations were applied to the DRG culture in different cell concentrations and combinations, and compared to medium. After 24 hours of exposure, the neurite outgrowth was reassessed and expressed as the ratio between the outgrowth at 48 and 24 hours culture.
NC in intermediate and high concentration and CC in high concentration induced a significant inhibition of the neurite outgrowth compared to culture medium. Further, one of the combinations (low NC and high CC concentration) resulted in a significant inhibition of the neurite outgrowth.
The present study demonstrated negative effects of NP cells on nerve tissue culture explants. The combination of low NC and high CC concentrations may mimic the situation in humans, where we have an increased proportion of chondrocyte-like cells with age. The results from this study may provide a biologic explanation for the large variation of symptoms in disc herniation patients despite similar mechanical influence on nerve tissue.
一项实验研究，旨在探讨椎间盘细胞对大鼠背根神经节（DRG）培养系统中神经突生长的影响。
研究 2 种核髓核细胞群体（脊索细胞（NC）和软骨细胞样细胞（CC））对 DRG 神经突生长的影响。
NP 至少由 2 种细胞群体组成，即 NC 和 CC。NP 中的细胞已被证明对体外神经突生长和体内神经组织有负面影响。尚不清楚是 1 种细胞类型还是 2 种细胞类型的组合负责报告的影响。
从新生 Sprague Dawley 大鼠中采集 939 个 DRG 并置于培养皿中。24 小时后，测量神经突生长。从成年大鼠的尾盘中采集 NP，并将 NP 细胞分离成 2 个群体，NC 和 CC。将细胞群体以不同的细胞浓度和组合应用于 DRG 培养，并与培养基进行比较。暴露 24 小时后，再次评估神经突生长，并表示为 48 小时和 24 小时培养之间的生长比率。
与培养基相比，中等和高浓度的 NC 和高浓度的 CC 诱导神经突生长明显抑制。此外，一种组合（低 NC 和高 CC 浓度）导致神经突生长明显抑制。
本研究表明 NP 细胞对神经组织培养外植体有负面影响。低 NC 和高 CC 浓度的组合可能模拟了人类的情况，即随着年龄的增长，软骨细胞样细胞的比例增加。本研究的结果可能为椎间盘突出症患者尽管对神经组织有相似的机械影响，但症状差异较大提供生物学解释。
