Corallini A, Altavilla G, Cecchetti M G, Fabris G, Grossi M P, Balboni P G, Lanza G, Barbanti-Brodano G
J Natl Cancer Inst. 1978 Sep;61(3):875-83.
BK virus (BKV), a human papovavirus, was inoculated iv into 3-week-old Syrian golden hamsters. Between 2 1/2 and 9 months after inoculation, 82% of the animals developed tumors. The induced neoplasms were ependymoma, carcinoma of the pancreatic islets, osteosarcoma, adenocarcinoma, angiosarcoma, angioma, lymphoma, and seminoma. Hypersecretion of insulin, glucagon, C-peptide, and calcitonin was detected in tumors of pancreatic islets. BKV etiology of tumors was supported by the following evidence: 1) No tumors with BKV-specific markers appeared in animals given injections of buffer, animals inoculated with BKV neutralized by anti-BKV-specific serum, or uninoculated controls; 2) BKV tumor (T) antigen was detected by immunofluorescence and complement fixation tests in tumors of animals inoculated with infectious BKV and in transplanted tumors; 3) antibodies to BKV T-antigen were detected in sera of animals bearing primary or transplanted tumors; 4) BKV could be activated by Sendai virus-mediated fusion of neoplastic cells with susceptible Vero cells; and 5) no endogenous hamster oncornaviruses were found in tumors.
BK病毒（BKV）是一种人乳头瘤多瘤空泡病毒，通过静脉注射接种到3周龄的叙利亚金黄地鼠体内。接种后2个半月至9个月期间，82%的动物发生了肿瘤。诱发的肿瘤有室管膜瘤、胰岛癌、骨肉瘤、腺癌、血管肉瘤、血管瘤、淋巴瘤和精原细胞瘤。在胰岛肿瘤中检测到胰岛素、胰高血糖素、C肽和降钙素的分泌过多。肿瘤的BKV病因学得到以下证据支持：1）注射缓冲液的动物、接种被抗BKV特异性血清中和的BKV的动物或未接种的对照动物均未出现带有BKV特异性标记的肿瘤；2）通过免疫荧光和补体结合试验在接种传染性BKV的动物肿瘤和移植肿瘤中检测到BKV肿瘤（T）抗原；3）在患有原发性或移植性肿瘤的动物血清中检测到抗BKV T抗原的抗体；4）BKV可通过仙台病毒介导的肿瘤细胞与易感Vero细胞融合而被激活；5）在肿瘤中未发现内源性仓鼠肿瘤病毒。
