Sekiguchi M, Satomura T, Saëgusa M, Takeuchi H, Asanuma K, Shimoda T
Department of Clinical Oncology, University of Tokyo, Japan.
Int J Exp Pathol. 1994 Feb;75(1):51-60.
Animal models of osteosarcoma with spontaneous pulmonary metastasis which retain metastatic capacity and osteoid formation after serial passages have been reported infrequently. In this communication we describe some biological features of a transplantable osteosarcoma, Os515, induced by BK-virus in Syrian golden hamsters. The subcutaneously transplanted tumours in 2-week-old animals grew progressively until death, with a mean survival time of 32 days. Distant metastases occurred only in the lungs in all animals. The histological appearance was osteosarcoma of osteoblastic type. Enzyme-histochemical staining showed alkaline phosphatase activity in many cells and beta-glucuronidase activity in few cells. Tumours transplanted intramuscularly in the hind limbs were amputated radically at 5 or 11 days. A small number of animals died from lung metastases without local relapse during the observation period of 140 days after grafting. All the control hamsters bearing unamputated tumours died much earlier. Necropsy revealed large metastatic nodules in the lungs of limb-amputated animals and small diffuse nodules in the lungs of untreated control animals. The development of lung metastases was monitored by soft X-ray without sacrificing the animals. This model will be useful in studies of mechanisms of metastasis and for the experimental treatment of osteosarcoma.
具有自发肺转移且在连续传代后仍保留转移能力和类骨质形成的骨肉瘤动物模型鲜有报道。在本报告中，我们描述了一种由BK病毒在叙利亚金仓鼠中诱导产生的可移植性骨肉瘤Os515的一些生物学特性。在2周龄动物中皮下移植的肿瘤逐渐生长直至动物死亡，平均存活时间为32天。所有动物均仅在肺部发生远处转移。组织学表现为成骨细胞型骨肉瘤。酶组织化学染色显示许多细胞中有碱性磷酸酶活性，少数细胞中有β-葡萄糖醛酸酶活性。在后肢肌肉内移植的肿瘤在5天或11天时被根治性切除。少数动物在移植后140天的观察期内死于肺转移且无局部复发。所有携带未切除肿瘤的对照仓鼠死亡时间要早得多。尸检发现肢体切除动物的肺部有大的转移结节，未治疗的对照动物肺部有小的弥漫性结节。通过软X射线监测肺转移的发展而不牺牲动物。该模型将有助于骨肉瘤转移机制的研究以及骨肉瘤的实验性治疗。
