Institut de Recherche pour le Développement, Centre de Biologie pour la Gestion des Populations, Centre Régional Agrhymet, Niamey, Niger.
Acta Trop. 2011 Mar;117(3):183-8. doi: 10.1016/j.actatropica.2010.11.004. Epub 2010 Nov 30.
Invading rodent species can harbor parasites with potential transmission to native rodents and/or humans. To investigate trypanosomes prevalence in rodents, the spleen of 76 rodents from Niger identified by their karyotype was used as a DNA source for Trypanosoma detection using a newly developed qPCR assay. Of the invasive black rat, Rattus rattus, 71% (10/14) were PCR positive as well as 6% (4/62) of native African rodents. Sequences of ~400bp of the SSU rDNA gene identified phylogenetically close Trypanosoma lineages. Trypanosoma lewisi was present in all positive black rats and the sequences displayed 100% similarity with T. lewisi-infected humans in Senegal. T. lewisi was also detected in one Acomys johannis, suggesting a possible transmission to native species. In addition to improved knowledge of Trypanosoma diversity in rodents, our data underscore the introduction of the potentially pathogenic T. lewisi kinetoplastid through the human-mediated invasion of black rats all over West Africa.
入侵的啮齿动物物种可能携带寄生虫，这些寄生虫有可能传播给本地啮齿动物和/或人类。为了调查尼日尔啮齿动物中的锥体虫流行情况，使用新开发的 qPCR 检测法，从通过核型鉴定的 76 只啮齿动物的脾脏中提取 DNA 作为 DNA 来源，用于检测锥体虫。在入侵的黑鼠中，71%（14/14）的 PCR 检测呈阳性，而在 62 只本地非洲啮齿动物中，有 6%（4/62）呈阳性。~400bp 的 SSU rDNA 基因序列鉴定出与锥体虫亲缘关系密切的谱系。所有阳性黑鼠中均存在 Lewisi 锥体虫，其序列与塞内加尔感染人类的 Lewisi 锥体虫完全一致。在一只 Acomys johannis 中也检测到了 Lewisi 锥体虫，表明可能向本地物种传播。除了提高对啮齿动物锥体虫多样性的认识外，我们的数据还强调了通过人类介导的黑鼠入侵，将潜在致病性的 Lewisi 锥体虫引入整个西非。
