College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, 88 Wenhua East Road, Jinan 250014, People's Republic of China.
Biosens Bioelectron. 2011 Jan 15;26(5):2489-94. doi: 10.1016/j.bios.2010.10.040. Epub 2010 Oct 31.
A novel non-enzyme glucose amperometric biosensor was fabricated based on biospecific binding affinity of concanavalin A (Con A) for D-glucose on thionine (TH) modified electrode. TH can be covalently immobilized on potentiostatically activated glassy carbon electrode through Schiff-base reaction. Subsequently, the surface-adherent polydopamine film formed by self-polymerization of dopamine attached to TH and afforded binding sites for the subsequent immobilization of Con A molecules via Michael addition and/or Schiff-base reaction with high stability. Thus, a sensing platform for specific detection towards D-glucose was established. The binding of Con A towards D-glucose can be monitored through the decrease of the electrode response of the TH moiety. Due to the high affinity of Con A for D-glucose and high stability of the resulting sensing platform, the fabricated biosensor exhibited high selectivity, good sensitivity, and wide linear range from 1.0×10(-6) to 1.0×10(-4) M with a low detection limit of 7.5×10(-7) M towards D-glucose.
基于刀豆蛋白 A（Con A）对葡萄糖的生物特异性结合亲和力，构建了一种新型非酶葡萄糖电流型生物传感器。通过席夫碱反应，噻吩（TH）可以共价固定在恒电位激活的玻碳电极上。随后，多巴胺通过自聚合附着在 TH 上形成的表面附着的聚多巴胺薄膜，提供了用于随后通过迈克尔加成和/或席夫碱反应固定 Con A 分子的结合位点，具有高稳定性。因此，建立了一种用于特定检测 D-葡萄糖的传感平台。通过检测 TH 部分的电极响应的降低，可以监测 Con A 与 D-葡萄糖的结合。由于 Con A 与 D-葡萄糖的高亲和力和所得传感平台的高稳定性，所构建的生物传感器表现出高选择性、良好的灵敏度和宽线性范围，从 1.0×10(-6)到 1.0×10(-4) M，检测限低至 7.5×10(-7) M 对 D-葡萄糖。
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