Department of Chemistry, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada.
Biosens Bioelectron. 2011 Dec 15;30(1):287-93. doi: 10.1016/j.bios.2011.09.030. Epub 2011 Oct 1.
Here we report on a novel platform based on buckypaper for the design of high-performance electrochemical biosensors. Using glucose oxidase as a model enzyme, we constructed a biocompatible mediator-free biosensor and studied the potential effect of the buckypaper on the stability of the biosensor with both amperometry and FTIR spectroscopy. The results showed that the biosensor responses sensitively and selectively to glucose with a considerable functional lifetime of over 80 days. The fabricated enzymatic sensor detects glucose with a dynamic linear range of over 9 mM and a detection limit of 0.01 mM. To examine the efficiency of enzyme immobilization, the Michaelis-Menten constant (K(M)(app)) was calculated to be 4.67 mM. In addition, the fabricated electrochemical biosensor shows high selectivity; no amperometric response to the common interference species such as ascorbic acid, uric acid and acetamidophenol was observed. The facile and robust buckypaper-based platform proposed in this study opens the door for the design of high-performance electrochemical biosensors for medical diagnostics and environmental monitoring.
在这里，我们报告了一种基于巴基纸的新型平台，用于设计高性能电化学生物传感器。我们使用葡萄糖氧化酶作为模型酶，构建了一种具有生物相容性的无介体生物传感器，并通过电流法和傅里叶变换红外光谱法研究了巴基纸对生物传感器稳定性的潜在影响。结果表明，该生物传感器对葡萄糖具有敏感和选择性的响应，功能寿命超过 80 天。所制备的酶传感器检测葡萄糖的动态线性范围超过 9 mM，检测限为 0.01 mM。为了检验酶固定化的效率，计算得到米氏常数（K（M）（app））为 4.67 mM。此外，所制备的电化学生物传感器具有高选择性；对常见的干扰物质如抗坏血酸、尿酸和乙酰氨基酚没有电流响应。本研究中提出的简便而坚固的巴基纸基平台为用于医疗诊断和环境监测的高性能电化学生物传感器的设计开辟了道路。
