Xu Dongling, Wang Xingming, Fei Dan, Ding Lisheng
Department of Chemistry, Material Science and Engineering College, Southwest University of Science and Technology, Mianyang, Sichuan, P. R. China.
Nucleosides Nucleotides Nucleic Acids. 2010 Nov;29(11):854-66. doi: 10.1080/15257770.2010.531858.
Ultraviolet-visible (UV-vis) spectra, fluorescence spectra, electrochemistry, and the thermodynamic method were used to discuss the interaction mode between the inclusion complex of hematoxylin with β-cyclodextrin and herring sperm DNA. On the condition of physiological pH, the result showed that hematoxylin and β-cyclodextrin formed an inclusion complex with binding ratio n(hematoxylin):n(β-cyclodextrin) = 1:1. The interaction mode between β-cyclodextrin-hematoxylin and DNA was a mixed binding, which contained intercalation and electrostatic mode. The binding ratio between β-cyclodextrin-hematoxylin and DNA was n(β-cyclodextrin -hematoxylin):n(DNA) = 2:1, binding constant was K(⊖)(298.15K) = 5.29 × 10⁴ L·mol⁻¹, and entropy worked as driven force in this action.
利用紫外可见光谱、荧光光谱、电化学和热力学方法探讨了苏木精与β-环糊精包合物与鲱鱼精DNA之间的相互作用模式。在生理pH条件下，结果表明苏木精与β-环糊精形成了包合物，结合比为n(苏木精):n(β-环糊精)=1:1。β-环糊精-苏木精与DNA之间的相互作用模式为混合结合，包括插入和静电模式。β-环糊精-苏木精与DNA的结合比为n(β-环糊精-苏木精):n(DNA)=2:1，结合常数为K(⊖)(298.15K)=5.29×10⁴L·mol⁻¹，且熵在该作用中起驱动力作用。
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