School of Environmental Science and Engineering, Shandong University, Jinan 250100, People's Republic of China.
J Biochem Mol Toxicol. 2010 Sep-Oct;24(5):323-9. doi: 10.1002/jbt.20341.
The toxic interaction of melamine with herring sperm DNA (hs-DNA) was investigated by using fluorescence and UV-vis absorption spectra techniques. The experimental results showed that the toxic interaction between melamine and hs-DNA occurred. Fluorescence quenching experiments indicated the existence of electrostatic binding between melamine and hs-DNA. The binding constants K(A) and the binding site numbers were calculated by means of the Stern-Volmer equation and were 9.8 × 10(4) L mol(-1) and 1.3, respectively. Both the results of fluorescence spectra and UV-vis absorption spectra verified that there are electrostatic binding between melamine and hs-DNA. The possibility in the presence of a classical intercalation binding mode could be ruled out by using DNA unwinding experiments.
采用荧光光谱和紫外吸收光谱技术研究了三聚氰胺与鲱精 DNA（hs-DNA）的毒性相互作用。实验结果表明，三聚氰胺与 hs-DNA 之间发生了毒性相互作用。荧光猝灭实验表明三聚氰胺与 hs-DNA 之间存在静电结合。通过 Stern-Volmer 方程计算了结合常数 K(A)和结合位点数，分别为 9.8×10(4)L mol(-1)和 1.3。荧光光谱和紫外吸收光谱的结果均证实了三聚氰胺与 hs-DNA 之间存在静电结合。通过 DNA 解链实验排除了存在经典嵌入结合模式的可能性。
