Tang Yan, Cai Li, Xue Kang, Wang Chunling, Xiong Xiaoli
a College of Chemistry and Materials Science , Sichuan Normal University , Chengdu , P.R. China.
b College of Chemical Engineering , Sichuan University , Chengdu , P.R. China.
Nucleosides Nucleotides Nucleic Acids. 2016 May 3;35(5):245-58. doi: 10.1080/15257770.2016.1139125. Epub 2016 Apr 8.
Methods including spectroscopy, electronic chemistry and thermodynamics were used to study the inclusion effect between γ-cyclodextrin (CD) and vitamin K3(K3), as well as the interaction mode between herring-sperm DNA (hsDNA) and γ-CD-K3 inclusion complex. The results from ultraviolet spectroscopic method indicated that VK3 and γ-CD formed 1:1 inclusion complex, with the inclusion constant Kf = 1.02 × 10(4) L/mol, which is based on Benesi-Hildebrand's viewpoint. The outcomes from the probe method and Scatchard methods suggested that the interaction mode between γ-CD-K3 and DNA was a mixture mode, which included intercalation and electrostatic binding effects. The binding constants were K (θ)25°C = 2.16 × 10(4) L/mol, and K(θ)37°C = 1.06 × 10(4) L/mol. The thermodynamic functions of the interaction between γ-CD-K3 and DNA were ΔrHm(θ) = -2.74 × 10(4) J/mol, ΔrSm(θ) = 174.74 J·mol(-1)K(-1), therefore, both ΔrHm(θ) (enthalpy) and ΔrSm(θ) (entropy) worked as driven forces in this action.
采用光谱法、电化学法和热力学方法研究了γ-环糊精（CD）与维生素K3（K3）之间的包合作用，以及鲱鱼精DNA（hsDNA）与γ-CD-K3包合物之间的相互作用模式。紫外光谱法结果表明，基于贝内西-希尔德布兰德观点，VK3与γ-CD形成1:1包合物，包合常数Kf = 1.02×10⁴ L/mol。探针法和斯卡查德法结果表明，γ-CD-K3与DNA的相互作用模式为混合模式，包括嵌入和静电结合作用。结合常数为K(θ)25°C = 2.16×10⁴ L/mol，K(θ)37°C = 1.06×10⁴ L/mol。γ-CD-K3与DNA相互作用的热力学函数为ΔrHm(θ) = -2.74×10⁴ J/mol，ΔrSm(θ) = 174.74 J·mol⁻¹K⁻¹，因此，ΔrHm(θ)（焓）和ΔrSm(θ)（熵）在该作用中均作为驱动力。
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