Zhou Jian, Li Ya-Nan, Song Yong-Ping, Wei Xu-Dong, Guo Kun-Yuan, Wu Yuan-Bin
Department of Hematology, Henan Tumor Hospital, Henan Institute of Hematology, Zhengzhou 450003, Henan Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Oct;18(5):1128-31.
The purpose of study was to investigate the feasibility for establishing erythroleukemia model in CB6F1 mice by transplant with haploidentical mouse leukemic cell line FBL-3 and to explore the biological characteristics of FBL-3 cells in CB6F1 mice, CB6F1 and C57BL/6 mice were inoculated intravenously at doses of 1×10(3)-1×10(7) FBL-3 cells respectively. The survival time, the count of peripheral white blood cells, the percentage of erythroblasts and chromosome of these mice were observed. The liver, spleen, lung and kidney were obtained from the dying CB6F1 mice for pathological examination. The ultrastructure of erythroblasts in bone marrow and spleen was observed by transmission electron microscopy as soon as these mice died. Expression of MHC molecules and karyotype of spleen and bone marrow cell were measured. The results showed that 100% and 92.5% incidences of erythroleukemia were observed when 1×10(3)-1×10(7) FBL-3 cells had been administrated intravenously to CB6F1 and C57BL/6 mouse, respectively. There was a linear relationship between the survival time and the number of inoculated leukemic cells. The survival time of CB6F1 was longer than C57BL/6 mice inoculated the same number cell. The main targets for FBL-3 cell infiltration were liver, spleen, marrow, lung and kidney. The reaction of FBL-3 cells to glycogen staining was positive, while the to reaction peroxidase, alkaline phosphatase and butyric acid staining were negative, reaction to chloroacetic acid staining partially was positive. Virus-like particles were found in the spleen and bone marrow cells under electron microscope. Chromosomes of spleen and bone marrow cells in the majority were non-diploid, and the expression of H-2b increased, H-2d expression decreased. It is concluded that the erythroleukemia mouse model can be established in CB6F1 mice transplanted with leukemic FBL-3 cells, that provides a convenience experimental erythroleukemia model for study.
本研究旨在探讨通过移植半相合小鼠白血病细胞系FBL-3在CB6F1小鼠中建立红白血病模型的可行性，并探索FBL-3细胞在CB6F1小鼠中的生物学特性，分别以1×10(3)-1×10(7)个FBL-3细胞的剂量对CB6F1和C57BL/6小鼠进行静脉接种。观察这些小鼠的生存时间、外周血白细胞计数、幼红细胞百分比和染色体情况。从濒死的CB6F1小鼠获取肝脏、脾脏、肺和肾脏进行病理检查。这些小鼠死亡后，立即通过透射电子显微镜观察骨髓和脾脏中幼红细胞的超微结构。检测脾脏和骨髓细胞的MHC分子表达和核型。结果显示，分别向CB6F1和C57BL/6小鼠静脉注射1×10(3)-1×10(7)个FBL-3细胞时，红白血病的发生率分别为100%和92.5%。生存时间与接种白血病细胞数量之间存在线性关系。接种相同数量细胞时，CB6F1小鼠的生存时间比C57BL/6小鼠长。FBL-3细胞浸润的主要靶器官为肝脏、脾脏、骨髓、肺和肾脏。FBL-3细胞糖原染色反应呈阳性，而过氧化物酶、碱性磷酸酶和丁酸染色反应呈阴性，氯乙酸染色部分呈阳性。电镜下在脾脏和骨髓细胞中发现病毒样颗粒。大多数脾脏和骨髓细胞的染色体为非二倍体，H-2b表达增加，H-2d表达降低。结论是，移植白血病FBL-3细胞可在CB6F1小鼠中建立红白血病小鼠模型，为研究提供了便利的实验性红白血病模型。
