Institute of Veterinary Research of Tunisia, Tunis 1006, Tunisia.
Int J Food Microbiol. 2011 Jan 5;144(3):497-502. doi: 10.1016/j.ijfoodmicro.2010.11.008. Epub 2010 Nov 13.
The antimicrobial resistance phenotype and genotype, the flanking regions of sulphonamide resistance genes and the integrons were analyzed in 166 Escherichia coli isolates recovered from poultry meat in Tunisia. High percentages of resistance were detected to ampicillin, streptomycin, nalidixic acid, sulphonamide and tetracycline (66-95%), and lower percentages to gentamicin, amoxicillin-clavulanic acid and cefoxitin (1-4%). The bla(TEM), tet(A)/tet(B), aph(3')-Ia, aac(6')-Ib-cr, aac(3)-II and cmlA genes were identified in 92, 82, 29, 2, 2 and 7 isolates, respectively. Class 1 and/or class 2 integrons were detected in 52% of E. coli isolates and five different gene cassette arrangements were identified in the variable regions of class 1 integrons, which included antimicrobial resistance determinants. Sixty-eight isolates contained the sul1 gene and 37 of them presented this gene into a class 1 integron structure. The sul3 gene was detected associated with non-classic class 1 integrons in 4 out of 46 sul3-positive isolates. The sul2 gene was detected in 66 isolates, 51 of them were linked to strA/B genes in seven different genetic structures. Seventy-three-per-cent of integron-positive isolates presented resistance to at least five different antimicrobial families versus 38.7% of integron-negative isolates. Our study highlights the role of commensal E. coli isolates from poultry meat as an important reservoir for sulphonamide resistance genes and integrons carrying antimicrobial resistance genes.
从突尼斯禽肉中分离的 166 株大肠杆菌的抗菌药物耐药表型和基因型、磺胺类耐药基因侧翼区和整合子分析。检测到对氨苄西林、链霉素、萘啶酸、磺胺类和四环素的耐药率较高（66-95%），对庆大霉素、阿莫西林-克拉维酸和头孢西丁的耐药率较低（1-4%）。在 92、82、29、2、2 和 7 株分离株中分别鉴定出 bla(TEM)、tet(A)/tet(B)、aph(3')-Ia、aac(6')-Ib-cr、aac(3)-II 和 cmlA 基因。52%的大肠杆菌分离株中检测到 1 类和/或 2 类整合子，在 1 类整合子可变区中鉴定出 5 种不同的基因盒排列，其中包括抗菌药物耐药决定因素。68 株分离株含有 sul1 基因，其中 37 株呈 sul1 基因与 1 类整合子结构。在 46 株 sul3 阳性分离株中，有 4 株检测到与非经典 1 类整合子相关的 sul3 基因。在 66 株分离株中检测到 sul2 基因，其中 51 株与 7 种不同遗传结构中的 strA/B 基因相连。73%的整合子阳性分离株对至少 5 种不同的抗菌药物家族具有耐药性，而整合子阴性分离株的耐药率为 38.7%。我们的研究强调了来自禽肉的共生大肠杆菌分离株作为磺胺类耐药基因和携带抗菌药物耐药基因的整合子的重要储存库的作用。
