Institute of Applied Radiation Chemistry, Faculty of Chemistry, Technical University of Lodz, Wroblewskiego 15, 93-590 Lodz, Poland.
Colloids Surf B Biointerfaces. 2011 Mar;83(1):116-21. doi: 10.1016/j.colsurfb.2010.11.017. Epub 2010 Nov 17.
Interactions of two anionic surfactants, sodium dodecyl sulphate (SDS) and sodium bis(2-ethylhexyl) sulfosuccinate (AOT) at concentrations below and above critical micelle concentration with methemoglobin (metHb) have been investigated by conventional as well as by stopped-flow absorption and fluorescence spectroscopy. The absorption spectra of metHb in AOT reverse micelles have been also analyzed. Both surfactants in their monomeric form convert metHb to reversible hemichrome. This is connected with a diminution of peroxidase-like activity of metHb and with an increase of the susceptibility of heme for a damage by H(2)O(2). In micellar solutions of AOT and SDS as well as in AOT reverse micelles pentacoordinated ferric species seems to be the predominant form of this protein. It has been concluded, basing on a kinetic analysis, that conformational changes in the heme environment of metHb as induced by both surfactants occur independently of the alterations in the tertiary structure of this protein.
已经通过传统的吸收光谱和荧光光谱法以及停流吸收和荧光光谱法研究了两种阴离子表面活性剂，十二烷基硫酸钠（SDS）和双（2-乙基己基）磺基琥珀酸钠（AOT）在低于和高于临界胶束浓度下与高铁血红蛋白（metHb）的相互作用。还分析了 metHb 在 AOT 反胶束中的吸收光谱。两种表面活性剂在单体形式下都将 metHb 转化为可逆的亚铁血晶蛋白。这与 metHb 的过氧化物酶样活性降低以及血红素对 H（2）O（2）损伤的敏感性增加有关。在 AOT 和 SDS 的胶束溶液以及 AOT 反胶束中，五配位的三价铁物种似乎是该蛋白的主要形式。基于动力学分析得出结论，两种表面活性剂诱导的 metHb 血红素环境构象变化独立于该蛋白的三级结构的改变。
