Instituto de Sistemas Optoelectrónicos y Microtecnología, ETSI Telecomunicación, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Biosens Bioelectron. 2011 Jan 15;26(5):2801-4. doi: 10.1016/j.bios.2010.11.009. Epub 2010 Dec 4.
Micropatterned molecularly imprinted polymer (MIP) transmissive 2D diffraction gratings (DGs) are fabricated and evaluated as label-free antibiotic bio(mimetic)sensors. Polymeric gratings are prepared by using microtransfer molding based on SiO(2)/Si molds. The morphology of the MIP gratings is studied by optical and atomic force microscopes. MIP 2D-DGs exhibit 2D optical diffraction patterns, and measurement of changes in diffraction efficiency is used as sensor response. The refractive index of the micropatterned MIP material was estimated, via solvent index matching experiments, to be 1.486. Immersion of a MIP 2D-DG in different solutions of target-antibiotic enrofloxacin leads to significant variations in diffraction efficiency, demonstrating target-molecule detection. On the other hand, no significant response is observed for both control experiments: MIP grating exposed to a non-retained analyte and an equivalent non-imprinted polymer grating exposed to the target analyte, showing highly specific antibiotic label-free optical recognition.
微图案化分子印迹聚合物（MIP）透明二维衍射光栅（DG）被制备并作为无标记抗生素生物（模拟）传感器进行评估。基于 SiO 2 / Si 模具的微转移模塑用于制备聚合物光栅。使用光学和原子力显微镜研究 MIP 光栅的形态。MIP 2D-DG 表现出 2D 光学衍射图案，并且测量衍射效率的变化用作传感器响应。通过溶剂折射率匹配实验，估计微图案化 MIP 材料的折射率为 1.486。将 MIP 2D-DG 浸入不同浓度的目标抗生素恩诺沙星溶液中会导致衍射效率发生显著变化，从而实现对目标分子的检测。另一方面，对于两种对照实验都没有观察到明显的响应：暴露于未保留分析物的 MIP 光栅和暴露于目标分析物的等效非印迹聚合物光栅，表现出高度特异性的抗生素无标记光学识别。
