工作于过渡模式的长周期光栅作为无标记生物传感的有前景的技术平台。

Long period grating working in transition mode as promising technological platform for label-free biosensing.

作者信息

Pilla P, Manzillo P Foglia, Malachovska V, Buosciolo A, Campopiano S, Cutolo A, Ambrosio L, Giordano M, Cusano A

机构信息

Optoelectonic Division, Engineering Department, University of Sannio, Benevento, Italy.

出版信息

Opt Express. 2009 Oct 26;17(22):20039-50. doi: 10.1364/OE.17.020039.

DOI:10.1364/OE.17.020039

PMID:19997228

Abstract

We present the development of a platform for label-free biosensing based on overlayered Long Period Gratings (LPGs) working in transition mode. Nano-scale layers of Polystyrene (PS) with different thicknesses were deposited onto the same LPG to test the performances of the device in different working points of its modified sensitivity characteristic. Adsorption dynamic of biotinylated bovine serum albumin (BBSA) onto the PS overlays was on-line monitored as well as a subsequent streptavidin (SA) binding dynamic on the biotinylated sites of the protein ad-layer. Experimental results show that overlayered LPGs are among the most sensitive refractive index transducers to be employed in label-free biochemical detection and that wide margins of further optimization exist.

摘要

我们展示了一种基于工作在过渡模式的叠加长周期光栅（LPG）的无标记生物传感平台的开发。将不同厚度的纳米级聚苯乙烯（PS）层沉积到同一LPG上，以测试该器件在其修改后的灵敏度特性的不同工作点下的性能。在线监测生物素化牛血清白蛋白（BBSA）在PS覆盖层上的吸附动力学以及随后链霉亲和素（SA）在蛋白质吸附层的生物素化位点上的结合动力学。实验结果表明，叠加LPG是用于无标记生化检测的最灵敏的折射率传感器之一，并且存在进一步优化的广阔空间。