中国肇东盐碱地中分离到的一株耐盐细菌，命名为肇东盐单胞菌（Halomonas zhaodongensis）。
Halomonas zhaodongensis sp. nov., a slightly halophilic bacterium isolated from saline-alkaline soils in Zhaodong, China.
机构信息
Department of Microbiology and Biotechnology, Key Laboratory of Soybean Biology of Ministry of Education, and Key Laboratory of Agricultural Biological Functional Genes of Heilongjiang Province, Northeast Agricultural University, Harbin, 150030, People's Republic of China,
出版信息
Antonie Van Leeuwenhoek. 2013 Nov;104(5):685-94. doi: 10.1007/s10482-013-9976-3. Epub 2013 Jul 23.
A slightly halophilic bacterium (strain NEAU-ST10-25(T)) was isolated from saline-alkaline soils in Zhaodong City, Heilongjiang Province, China. The strain is a Gram-negative, aerobic motile rod. It accumulates poly-β-hydroxyalkanoate and produces exopolysaccharide. It produces beige-yellow colonies. Growth occurs at NaCl concentrations (w/v) of 0-15 % (optimum 3 %), at temperatures of 4-60 °C (optimum 35 °C) and at pH 6-12 (optimum pH 9). Its G+C content is 53.8 mol%. Phylogenetic analyses based on the separate 16S rRNA gene and concatenation of the 16S rRNA, gyrB and rpoD genes indicate that it belongs to the genus Halomonas in the class Gammaproteobacteria. The most phylogenetically related species is Halomonas alkaliphila DSM 16354(T), with which strain NEAU-ST10-25(T) showed 16S rRNA, gyrB and rpoD gene sequence similarities of 99.2, 82.3 and 88.2 %, respectively. The results of DNA-DNA hybridization assays showed 60.47 ± 0.69 % DNA relatedness between strain NEAU-ST10-25(T) and H. alkaliphila DSM 16354(T), 42.43 ± 0.37 % between strain NEAU-ST10-25(T) and Halomonas venusta DSM 4743(T) and 30.62 ± 0.43 % between strain NEAU-ST10-25(T) and Halomonas hydrothermalis DSM 15725(T). The major fatty acids are C18:1 ω7c (62.3 %), C16:0 (17.6 %), C16:1 ω7c/C16:1 ω6c (7.7 %), C14:0 (2.9 %), C12:0 3-OH (2.8 %), C10:0 (2.1 %) and C18:1 ω9c (1.6 %) and the predominant respiratory quinone is ubiquinone 9 (Q-9). The proposed name is Halomonas zhaodongensis, NEAU-ST10-25(T) (=CGMCC 1.12286(T) = DSM 25869(T)) being the type strain.
一株耐盐嗜碱菌（NEAU-ST10-25(T) 株）从中国黑龙江肇东市的盐碱土壤中分离得到。该菌株为革兰氏阴性、需氧运动杆状菌。它可以积累聚-β-羟基烷酸酯并产生胞外多糖。它产生浅黄褐色的菌落。生长在 NaCl 浓度（w/v）为 0-15%（最佳 3%）、温度为 4-60°C（最佳 35°C）和 pH 值为 6-12（最佳 pH 值 9）的环境中。其 G+C 含量为 53.8 mol%。基于 16S rRNA 基因和 16S rRNA、gyrB 和 rpoD 基因的连接体的系统发育分析表明，它属于γ-变形菌纲的盐单胞菌属。最具系统发育关系的物种是 Halomonas alkaliphila DSM 16354(T)，与 NEAU-ST10-25(T) 株相比，其 16S rRNA、gyrB 和 rpoD 基因序列相似性分别为 99.2%、82.3%和 88.2%。DNA-DNA 杂交试验结果表明，NEAU-ST10-25(T) 株与 H. alkaliphila DSM 16354(T) 之间的 DNA 同源性为 60.47±0.69%，与 Halomonas venusta DSM 4743(T) 之间的 DNA 同源性为 42.43±0.37%，与 Halomonas hydrothermalis DSM 15725(T) 之间的 DNA 同源性为 30.62±0.43%。主要脂肪酸为 C18:1 ω7c（62.3%）、C16:0（17.6%）、C16:1 ω7c/C16:1 ω6c（7.7%）、C14:0（2.9%）、C12:0 3-OH（2.8%）、C10:0（2.1%）和 C18:1 ω9c（1.6%），主要呼吸醌为泛醌 9（Q-9）。建议的名称为肇东盐单胞菌，NEAU-ST10-25(T)（=CGMCC 1.12286(T)=DSM 25869(T)）为模式菌株。
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