State Key Joint Laboratory on Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
The Key Laboratory for Microbial Resources of Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Int J Syst Evol Microbiol. 2012 Jan;62(Pt 1):173-178. doi: 10.1099/ijs.0.025627-0. Epub 2011 Mar 4.
A Gram-negative, aerobic, short rod-shaped and non-motile bacterium, strain A-1(T), was isolated from a saline soil contaminated with crude oil in Xianhe, Shangdong Province, China. Strain A-1(T) formed yellow colonies, was moderately halophilic and grew with 0.05-27.5% (w/v) total salts (optimum 5-8%), at 10-42 °C (optimum 30 °C) and at pH 5.5-9.0 (optimum pH 7.2). The dominant fatty acids (>5%) were C(16:0), summed feature 3 (comprising C(16:1)ω7c and/or iso-C(15:0) 2-OH), C(18:1)ω7c, C(19:0) cyclo ω8c and C(12:0) 3-OH and the predominant ubiquinone was Q-9. The genomic DNA G+C content was 67.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A-1(T) belonged to the genus Halomonas in the class Gammaproteobacteria. The closest relatives were Halomonas lutea YIM 91125(T) (97.7% 16S rRNA gene sequence similarity), H. muralis LMG 20969(T) (95.6%), H. pantelleriensis AAP(T) (95.5%) and H. kribbensis BH843(T) (95.2%). DNA-DNA relatedness between strain A-1(T) and H. lutea CCTCC AB 206093(T) was 27±3%. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain A-1(T) should be placed in the genus Halomonas as a representative of a novel species. The name Halomonas xianhensis sp. nov. is proposed, with strain A-1(T) (=CGMCC 1.6848(T) =JCM 14849(T)) as the type strain.
一株革兰氏阴性、需氧、短杆状且无动力的细菌，菌株 A-1(T)，从中国山东省仙河受原油污染的盐土中分离得到。菌株 A-1(T)形成黄色菌落，中度嗜盐，在 0.05-27.5%（w/v）总盐（最佳 5-8%）、10-42°C（最佳 30°C）和 pH5.5-9.0（最佳 pH7.2）条件下生长。优势脂肪酸（>5%）为 C(16:0)、特征脂肪酸 3（包含 C(16:1)ω7c 和/或 iso-C(15:0) 2-OH）、C(18:1)ω7c、C(19:0) 环 ω8c 和 C(12:0) 3-OH，主要的醌为 Q-9。基因组 DNA G+C 含量为 67.1mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 A-1(T)属于γ-变形菌纲的盐单胞菌属。与其亲缘关系最近的菌株为黄盐单胞菌 YIM 91125(T)（97.7% 16S rRNA 基因序列相似性）、盐壁单胞菌 LMG 20969(T)（95.6%）、潘泰莱里亚盐单胞菌 AAP(T)（95.5%）和克里布斯盐单胞菌 BH843(T)（95.2%）。菌株 A-1(T)与黄盐单胞菌 CCTCC AB 206093(T)的 DNA-DNA 同源性为 27±3%。根据表型、化学分类和系统发育特征，菌株 A-1(T)应归入盐单胞菌属，代表一个新的种。现提议将其命名为仙河盐单胞菌 sp. nov.，其模式菌株为 A-1(T)=CGMCC 1.6848(T)=JCM 14849(T)。
