Lethbridge Research Center, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada, T1J 4B1.
Arch Microbiol. 2011 Mar;193(3):169-77. doi: 10.1007/s00203-010-0654-9. Epub 2010 Dec 4.
The aim of this study was to isolate a novel bacterial strain with strong and broad spectrum antibacterial activity from a livestock feed prebiotic supplement. A novel strain, termed Paenibacillus polymyxa JB05-01-1, was isolated using traditional microbiological methods and identified on the basis of its phenotypic and biochemical properties as well as its 16S rRNA gene sequence. This strain was able to inhibit growth of gram-negative bacteria including Escherichia coli RR1, Pseudomonas fluorescens R73, Pantoea agglomerans BC1, Butyrivibrio fibrisolvens OR85, and Fibrobacter succinogenes. The above antagonism against the aforementioned bacteria was attributed to production of an antimicrobial substance(s) termed "JB05-01-1." Its production was optimal during the stationary phase. JB05-01-1 has a molecular weight of 2.5 KDa, its mode of action is bactericidal, and the divalent cations, Ca(2+) and Mn(2+), reduced its lethality. The antibacterial activity was heat-stable and was effective at a pH range of 2-9. Enzymes like trypsin, α-chymotrypsin, and proteinase K have abolished the antibacterial activity of JB05-01-1 indicating a proteinaceous motif. This type of naturally occurring bacteria and inhibitory substance(s) could represent an additional value in livestock feed supplements. The natural presence of antibacterial activity indicates an opportunity to decrease the addition of antibiotics.
本研究旨在从一种家畜饲料益生元补充剂中分离出具有强广谱抗菌活性的新型细菌菌株。使用传统的微生物学方法分离出一种新型菌株，命名为多粘类芽孢杆菌 JB05-01-1，并根据其表型和生化特性以及 16S rRNA 基因序列进行鉴定。该菌株能够抑制革兰氏阴性菌的生长，包括大肠杆菌 RR1、荧光假单胞菌 R73、成团泛菌 BC1、纤维丁酸弧菌 OR85 和产琥珀酸纤维梭菌。上述对上述细菌的拮抗作用归因于产生一种称为“JB05-01-1”的抗菌物质。其最佳产量在静止期。JB05-01-1 的分子量为 2.5 kDa，其作用模式为杀菌，二价阳离子 Ca(2+)和 Mn(2+)降低了其致死率。抗菌活性热稳定，在 pH 值为 2-9 的范围内有效。像胰蛋白酶、α-糜蛋白酶和蛋白酶 K 这样的酶已经消除了 JB05-01-1 的抗菌活性，表明存在蛋白结构。这种天然存在的细菌和抑制物质可能代表家畜饲料补充剂的额外价值。天然存在的抗菌活性表明有机会减少抗生素的添加。
