Department of Laboratory Animal Science, College of Basic Medical Sciences Third Military Medical University, 30 Gaotanyan Street, Chongqin 400038, China.
Curr Microbiol. 2011 Apr;62(4):1107-12. doi: 10.1007/s00284-010-9833-9. Epub 2010 Dec 5.
Human flora-associated (HFA) mice are frequently applied in studying the ecology and metabolism of human gut microbiota. However, the development and stability of the genus Bacteriodes, a prominent bacteria group of human gut microbiota, in HFA mice have not yet fully been examined. In this study, PCR-denaturing gradient gel electrophoresis (DGGE) analysis was employed to monitor the Bacteriodes community in the fecal microbiota of six HFA Kunming mice during a period of 3 weeks. Based on the DGGE banding patterns, the majority of prominent bands in the HFA mice DGGE profile were also typical bands in the human DGGE profile, despite the absence of three bands (corresponding to two different B. thetaiotaomicron strains and one B. intestinalis strain) from the human DGGE profile. The Dice coefficient of similarity for the fecal microbiota of HFA mice in comparison to the human donor sample ranged between 74 ± 6% and 81 ± 7%. The phylogeny of bands in the DGGE profile showed that the dominant Bacteriodes species in the fecal microbiota of HFA mice were B. thetaiotaomicron, representing 66.7% of all bands. Our results indicate that the genus Bacteriodes in the fecal microbiota of HFA mice was selected from the human donor and could remain relatively stable over time.
人源菌群定植（HFA）小鼠常用于研究人类肠道微生物群的生态学和代谢。然而，HFA 小鼠中厚壁菌门（Bacteriodes）这一重要的人类肠道微生物群属的发育和稳定性尚未得到充分研究。本研究采用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析方法，监测了 6 只 HFA 昆明小鼠粪便微生物群中厚壁菌门的变化情况，历时 3 周。根据 DGGE 条带图谱，HFA 小鼠 DGGE 图谱中的大多数主要条带也是人类 DGGE 图谱中的典型条带，尽管人类 DGGE 图谱中不存在 3 条带（对应于两种不同的 Bacteriodes thetaiotaomicron 菌株和一种 Bacteriodes intestinalis 菌株）。与人类供体样本相比，HFA 小鼠粪便微生物群的 Dice 相似系数在 74±6%至 81±7%之间。DGGE 图谱中条带的系统发育分析表明，HFA 小鼠粪便微生物群中的优势厚壁菌门物种是 Bacteriodes thetaiotaomicron，占所有条带的 66.7%。我们的研究结果表明，HFA 小鼠粪便微生物群中的厚壁菌门是从人类供体中选择出来的，并能在较长时间内保持相对稳定。
