Kibe Ryoko, Sakamoto Mitsuo, Yokota Hiroshi, Ishikawa Hiroki, Aiba Yuji, Koga Yasuhiro, Benno Yoshimi
Department of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Appl Environ Microbiol. 2005 Jun;71(6):3171-8. doi: 10.1128/AEM.71.6.3171-3178.2005.
Human flora-associated (HFA) mice have been considered a tool for studying the ecology and metabolism of intestinal bacteria in humans, although they have some limitations as a model. Shifts in dominant species of microbiota in HFA mice after the administration of human intestinal microbiota was revealed by 16S rRNA gene sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses. Characteristic terminal restriction fragments (T-RFs) were quantified as the proportion of total peak area of all T-RFs. Only the proportion of the T-RF peak at bp 366, identified as the Gammmaproteobacteria group and the family Coriobacteriaceae, was reduced in this study. Increased T-RFs over time at bp 56, 184, and 196 were affiliated with the Clostridium group. However, most of the isolated bacteria with unique population shifts were phylotypes. The vertical transmission of the intestinal microbiota of the mouse offspring was also investigated by dendrogram analysis derived from the similarity of T-RFLP patterns among samples. As a result, the intestinal microbiota of HFA mice and their offspring reflected the composition of individual human intestinal bacteria with some modifications. Moreover, we revealed that human-derived lactobacilli (HDL), which have been considered difficult to colonize in the HFA mouse intestine in previous studies based on culture methods, could be detected in the HFA mouse intestine by using a lactic acid bacterium-specific primer and HDL-specific primers. Our results indicate that the intestinal microbiota of HFA mice represents a limited sample of bacteria from the human source and are selected by unknown interactions between the host and bacteria.
人源菌群关联（HFA）小鼠被认为是研究人类肠道细菌生态和代谢的一种工具，尽管作为一种模型它存在一些局限性。通过16S rRNA基因序列和末端限制性片段长度多态性（T-RFLP）分析揭示了给HFA小鼠接种人类肠道微生物群后其微生物群优势物种的变化。特征性末端限制性片段（T-RFs）被量化为所有T-RFs总峰面积的比例。在本研究中，只有被鉴定为γ-变形菌纲和科里杆菌科的位于366 bp处的T-RF峰的比例降低了。随着时间推移，位于56、184和196 bp处T-RFs的增加与梭菌属相关。然而，大多数具有独特种群变化的分离细菌是系统发育型。还通过基于样本间T-RFLP模式相似性的树形图分析研究了小鼠后代肠道微生物群的垂直传播。结果，HFA小鼠及其后代的肠道微生物群反映了个体人类肠道细菌的组成，但有一些改变。此外，我们发现，基于培养方法的先前研究认为难以在HFA小鼠肠道中定殖的人源乳酸杆菌（HDL），通过使用乳酸菌特异性引物和HDL特异性引物可在HFA小鼠肠道中检测到。我们的结果表明，HFA小鼠的肠道微生物群代表了来自人类来源的有限细菌样本，并且是由宿主与细菌之间未知的相互作用所选择的。
