Food Safety Research Division, Korea Food Research Institute, Seoul 463-420, Korea.
J Food Prot. 2005 Aug;68(8):1743-7. doi: 10.4315/0362-028x-68.8.1743.
Aerosolized sanitizer was investigated as a potential alternative to aqueous and gaseous sanitizers for produce. Peroxyacetic acid was aerosolized (5.42 to 11.42 microm particle diameter) by a commercially available nebulizer into a model cabinet. Iceberg lettuce leaves were inoculated with three strains each of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium and then treated with aerosolized peroxyacetic acid for 10, 30, or 60 min in a model aerosol cabinet at room temperature (22 +/- 2 degrees C). After treatment, surviving healthy and injured bacterial cells were enumerated on appropriate selective agars or using the overlay agar method. Inoculated iceberg lettuce leaves exposed to aerosolized peroxyacetic acid for 10 min exhibited a 0.8-log reduction in E. coli O157:H7, a 0.3-log reduction in Salmonella Typhimurium, and a 2.5-log reduction in L. monocytogenes when compared with the control. After 30 min of treatment, the three pathogens were reduced by 2.2, 3.3, and 2.7 log, and after 60 min, the reductions were 3.4, 4.5, and 3.8 log, respectively. Aerosolization may be a new and convenient method for sanitizing produce for storage or shipping.
研究了气溶胶消毒剂作为水基和气体消毒剂的替代品在农产品中的应用。过氧乙酸（粒径 5.42 至 11.42 微米）通过市售的雾化器雾化到模型柜中。将冰生菜叶用三种菌株的大肠杆菌 O157:H7、单核细胞增生李斯特菌和肠炎沙门氏菌分别接种，然后在室温（22 +/- 2 摄氏度）下在模型气溶胶柜中用气溶胶过氧乙酸处理 10、30 或 60 分钟。处理后，在适当的选择性琼脂平板上或使用覆盖琼脂法对存活的健康和受伤的细菌细胞进行计数。与对照组相比，用气溶胶过氧乙酸处理 10 分钟的接种冰生菜叶使大肠杆菌 O157:H7减少了 0.8 对数，肠炎沙门氏菌减少了 0.3 对数，单核细胞增生李斯特菌减少了 2.5 对数。处理 30 分钟后，三种病原体分别减少了 2.2、3.3 和 2.7 对数，处理 60 分钟后，减少了 3.4、4.5 和 3.8 对数。气溶胶化可能是一种新的、方便的储存或运输农产品消毒方法。
