Laboratory of Fish Diseases, Department of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
Fish Shellfish Immunol. 2011 Feb;30(2):509-14. doi: 10.1016/j.fsi.2010.11.030. Epub 2010 Dec 4.
In this study, we aimed to identify the leukocyte population that expresses Fas ligand (FasL) in the Japanese flounder (Paralichthys olivaceus). The transcriptional activity of FasL was examined for the first time in the fish leukocytes. Transcription of the FasL gene in flounder leukocytes was significantly increased by phytohemagglutinin (PHA) treatment. All the leukocyte populations we tested possessed binding activity for PHA, but this was especially high in the lymphocyte population. However, the lymphocytes consisted of two subsets showing heterogeneity with respect to PHA binding, with the high-binding subset being surface IgM-negative. We also found that only the lymphocyte population showed a significant increase in the expression of the FasL gene after stimulation with PHA. In addition, only the lymphocyte subset showing high binding to PHA showed conspicuous expression of the FasL gene. This subset also had a CD3γ/δ+, CD8α+ and IgM heavy-chain (-) phenotype. These results suggested that lymphocytes with T-cell-like properties are FasL-expressing cells in the Japanese flounder.
在这项研究中，我们旨在确定在日本牙鲆（Paralichthys olivaceus）中表达 Fas 配体（FasL）的白细胞群体。首次在鱼类白细胞中检测到 FasL 的转录活性。植物血凝素（PHA）处理显著增加了牙鲆白细胞中 FasL 基因的转录。我们测试的所有白细胞群体都具有与 PHA 的结合活性，但在淋巴细胞群体中这种活性特别高。然而，淋巴细胞由两个亚群组成，在 PHA 结合方面表现出异质性，高结合亚群表面 IgM 阴性。我们还发现，只有淋巴细胞群体在 PHA 刺激后 FasL 基因的表达显著增加。此外，只有表现出高 PHA 结合的淋巴细胞亚群表现出 FasL 基因的明显表达。该亚群还具有 CD3γ/δ+、CD8α+和 IgM 重链（-）表型。这些结果表明，具有 T 细胞样特性的淋巴细胞是日本牙鲆中 FasL 表达细胞。
