Joy R W, Thorpe T A
Department of Biological Sciences, University of Calgary, Alberta, Canada.
In Vitro Cell Dev Biol. 1990 Jun;26(6):643-6. doi: 10.1007/BF02624215.
The effects of excision, light and cytokinin (N6-benzyladenine) on 14C-acetate metabolism in cotyledons of Pinus radiata (D. Don) were determined. 14CO2 was released and the distribution of radioactivity into lipids, sugars, organic acids and amino acids was determined. While light and cytokinin generally caused some increase in metabolism, the effect of excision, i.e., wounding, was most pronounced. Specific metabolites examined (citrate, malate, succinate, alanine, aspartate, glutamate and glutamine) were at least 50% greater in 14C-labeling in excised cotyledons as compared to intact seedlings. This enhancement of wound metabolism would mask possible morphogenically-related changes occurring at that time.
测定了切除、光照和细胞分裂素（N6-苄基腺嘌呤）对辐射松（D. Don）子叶中14C-乙酸代谢的影响。测定了14CO2的释放以及放射性在脂质、糖类、有机酸和氨基酸中的分布。虽然光照和细胞分裂素通常会使代谢有所增加，但切除（即创伤）的影响最为显著。与完整幼苗相比，切除的子叶中所检测的特定代谢物（柠檬酸、苹果酸、琥珀酸、丙氨酸、天冬氨酸、谷氨酸和谷氨酰胺）的14C标记至少高出50%。创伤代谢的这种增强会掩盖当时可能发生的与形态发生相关的变化。
