CICS-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Covilhã, Portugal.
Nanotechnology. 2011 Jan 7;22(1):015101. doi: 10.1088/0957-4484/22/1/015101. Epub 2010 Dec 6.
The encapsulation of DNA inside nanoparticles meant for gene delivery applications is a challenging process where several parameters need to be modulated in order to design nanocapsules with specific tailored characteristics. The purpose of this study was to investigate and improve the formulation parameters of plasmid DNA (pDNA) loaded in chitosan nanocapsules using tripolyphosphate (TPP) as polyanionic crosslinker. Nanocapsule morphology and encapsulation efficiency were analyzed as a function of chitosan degree of deacetylation and chitosan-TPP ratio. The manipulation of these parameters influenced not only the particle size but also the encapsulation and release of pDNA. Consequently the transfection efficiency of the nanoparticulated systems was also enhanced with the optimization of the particle characteristics. Overall, the differently formulated nanoparticulated systems possess singular properties that can be employed according to the desired gene delivery application.
用于基因传递应用的纳米粒子中 DNA 的包裹是一个具有挑战性的过程，需要调节多个参数，以便设计具有特定定制特性的纳米胶囊。本研究的目的是研究和改进壳聚糖纳米胶囊中负载的质粒 DNA（pDNA）的配方参数，使用三聚磷酸（TPP）作为聚阴离子交联剂。纳米胶囊的形态和包封效率作为壳聚糖脱乙酰度和壳聚糖-TPP 比的函数进行分析。这些参数的操纵不仅影响粒径，而且影响 pDNA 的包封和释放。因此，纳米颗粒系统的转染效率也随着颗粒特性的优化而提高。总的来说，不同配方的纳米颗粒系统具有独特的性质，可以根据所需的基因传递应用进行选择。
