Miklos G L, De Couet H G
Molecular Neurobiology Group, Research School of Biological Sciences, Australian National University, Canberra ACT.
J Neurogenet. 1990 Apr;6(3):133-51. doi: 10.3109/01677069009107106.
By using a well defined panel of chromosomal deficiencies, duplications and lethals, we have mapped three mutations causing flightlessness, flightless-I3, flightless-O2 and standby, to a single lethal complementation group (termed W-2) at the base of the X-chromosome of D. melanogaster. We also show that a fourth flightless mutation, termed grounded, previously mapped near to the base of the X-chromosome, is distal to the cytogenetic interval 18F to 20F. Mutants homozygous for the flightless-I3, flightless-O2 and standby mutations exhibit abnormalities of myofibrillar arrangements in the indirect flight muscles. They have distorted Z-bands and the myofibrils are often displaced from their normal parallel arrangement. These viable flightless mutations are all hypomorphs since the homozygous deficiency of the W-2 X-chromosomal region is lethal to the organism.
通过使用一组定义明确的染色体缺失、重复和致死突变体，我们已将导致无法飞行的三个突变，即flightless-I3、flightless-O2和standby，定位到黑腹果蝇X染色体基部的一个单一致死互补群（称为W-2）。我们还表明，第四个无法飞行的突变体，称为grounded，先前定位在X染色体基部附近，位于细胞遗传学区间18F至20F的远端。flightless-I3、flightless-O2和standby突变的纯合突变体在间接飞行肌中表现出肌原纤维排列异常。它们的Z带扭曲，肌原纤维常常偏离其正常的平行排列。这些存活的无法飞行的突变都是亚效等位基因，因为W-2 X染色体区域的纯合缺失对生物体是致死的。
