Davis M B, MacIntyre R J
Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
Genetics. 1988 Nov;120(3):755-66. doi: 10.1093/genetics/120.3.755.
The gene for alpha-glycerophosphate oxidase, the nuclear encoded mitochondrial enzyme of the alpha-glycerophosphate cycle (alpha GP); has been mapped in Drosophila melanogaster. Several interstitial deficiencies in region 50c-53AB of chromosome 2R were used to localize the structural gene to 52D2-5. In addition, mutations of alpha GPO were generated; alpha GPO mutants are viable yet flightless. Interactions of alpha GPO with alpha-glycerophosphate dehydrogenase (alpha GPDH), the cytoplasmic enzyme of the alpha GP cycle, were investigated through the synthesis of a series of alpha GPDHnull-alpha GPOnull double mutants. Of the six double null mutants constructed, four alpha GPDH-alpha GPO double nulls are viable and flightless. Two double mutants, however, exhibit an allelic-dependent synthetic lethal phenotype.
α-甘油磷酸氧化酶基因，即α-甘油磷酸循环（α GP）的核编码线粒体酶，已在黑腹果蝇中定位。利用2R染色体50c - 53AB区域的几个中间缺失来将结构基因定位到52D2 - 5。此外，还产生了α GPO的突变体；α GPO突变体能够存活但无法飞行。通过合成一系列α GPDH缺失-α GPO缺失双突变体，研究了α GPO与α-甘油磷酸脱氢酶（α GPDH，α GP循环的细胞质酶）之间的相互作用。在构建的六个双缺失突变体中，四个α GPDH-α GPO双缺失突变体能够存活但无法飞行。然而，有两个双突变体表现出等位基因依赖性的合成致死表型。
