Department of Animal Biotechnology and Cell Engineering, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science, Wuhan 430065, People's Republic of China.
In Vitro Cell Dev Biol Anim. 2011 Mar;47(3):188-94. doi: 10.1007/s11626-010-9376-1. Epub 2010 Dec 7.
Embryonic stem (ES) cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES cells to improve functional outcome following mammary gland injury. This study investigates the feasibility of implanting mouse ES cells labeled with enhanced green fluorescence protein in the developing mammary glands in order to acquire lineage-committed cells in mammary (mammary gland epithelial cell or luminal cell). Cells implanted in high numbers (5 × 10(6) cells per mammary gland) survived in the majority of the mice and nearly 38.4% of the surviving cells were CK18(+) at 15th week following the transplantation. Our results may provide a technique instrument on advanced therapy of breast diseases and the mammary regeneration after breast ablated partly.
胚胎干细胞（ES 细胞）已在许多严重损伤和退行性疾病的动物模型中进行了研究。然而，很少有研究探讨 ES 细胞改善乳腺损伤后功能结果的能力。本研究探讨了在发育中的乳腺中植入标记有增强型绿色荧光蛋白的小鼠 ES 细胞以获得乳腺（乳腺上皮细胞或腔细胞）中谱系定向细胞的可行性。以高数量（每乳腺 5×10(6)个细胞）植入的细胞在大多数小鼠中存活，并且在移植后第 15 周，近 38.4%的存活细胞为 CK18(+)。我们的结果可能为乳腺疾病的先进治疗和乳腺切除后部分再生提供技术手段。
