de Spíndula-Filho José Vieira, da Cruz Aparecido Divino, Oton-Leite Angélica Ferreira, Batista Aline Carvalho, Leles Cláudio Rodrigues, de Cássia Gonçalves Alencar Rita, Saddi Vera Aparecida, Mendonça Elismauro Francisco
Department of Stomatology (Oral Pathology), Dental School, Federal University of Goiás, Goiás, Brazil.
Tumour Biol. 2011 Apr;32(2):409-16. doi: 10.1007/s13277-010-0135-4. Epub 2010 Dec 7.
Human papillomavirus (HPV) has been cited as a possible initiating agent in the pathogenesis of oral cancer. However, the literature tends to be both controversial and inconclusive about the prevalence of HPV and its potential for proliferation in oral squamous cell carcinoma (SCC). The aim of this study was to investigate the cellular proliferation and the presence of HPV in SCC and verrucous carcinoma (VC). Forty-seven samples of SCC were selected and divided into three groups: 39 SCC, 8 VC, and 9 of normal mucosa (control-CT). Quantitative analyses of all groups showed a greater expression of PCNA, followed by Ki-67 and cyclin B1. A significant difference was observed in cyclin B1 expression in the SCC group compared with VC. PCNA, Ki-67, and cyclin B1 were statistically significant when comparing the SCC and CT groups. However, when SCC and VC were compared, there was no difference in Ki-67 expression. Our results showed that only cyclin B1 had an association with histological grade, and that poorly differentiated tumors presented a higher expression of cyclin B1. Therefore, considerable differences in the cellular proliferation between SCC and VC were observed, and no correlation with HPV was established, since all samples were negative for HPV.
人乳头瘤病毒（HPV）被认为是口腔癌发病机制中一种可能的起始因子。然而，关于HPV在口腔鳞状细胞癌（SCC）中的患病率及其增殖潜力，文献往往存在争议且尚无定论。本研究的目的是调查SCC和疣状癌（VC）中的细胞增殖情况以及HPV的存在情况。选取了47例SCC样本并分为三组：39例SCC、8例VC以及9例正常黏膜（对照组-CT）。对所有组的定量分析显示，增殖细胞核抗原（PCNA）的表达更高，其次是Ki-67和细胞周期蛋白B1。与VC相比，SCC组中细胞周期蛋白B1的表达存在显著差异。比较SCC组和CT组时，PCNA、Ki-67和细胞周期蛋白B1具有统计学意义。然而，比较SCC和VC时，Ki-67的表达没有差异。我们的结果表明，只有细胞周期蛋白B1与组织学分级相关，且低分化肿瘤呈现出更高的细胞周期蛋白B1表达。因此，观察到SCC和VC之间在细胞增殖方面存在相当大的差异，且未发现与HPV相关，因为所有样本的HPV检测均为阴性。
