Internal Medicine, Human Genetics, Public Health and Center for Computational Medicine and Biology, University of Michigan, Ann Arbor, MI, USA.
Proteomics Clin Appl. 2007 Aug;1(8):769-79. doi: 10.1002/prca.200700369. Epub 2007 Jul 13.
The Human Proteomics Organization (HUPO) Human Plasma Proteome Project (PPP) is a prominent example of the inherently collaborative nature of the overall community effort to characterize the proteome of humans in health and disease. The PPP Pilot Phase, called "Exploring the Human Plasma Proteome", engaged 55 laboratories, four technical committees, and vendors and sponsors on an international scale. Among other outcomes, the PPP generated a Core Dataset of 3020 proteins identified with two or more peptides, fully accessible at EBI/PRIDE, ISB/PeptideAtlas, and University of Michigan websites, a rich resource for follow-on analyses. The PPP provided extensive annotation, correlation of number of peptides with protein concentrations measured by immunoassay, an algorithm for choice of a representative protein for multiple proteins matching a given peptide, and independent analyses from the raw spectra. The next phase of the PPP will emphasize standardized procedures for specimen handling, potent new technology platforms for discovery and for targeted proteomics, and robust informatics efforts, including comparative analyses of other biofluids.
人类蛋白质组组织（HUPO）人类血浆蛋白质组计划（PPP）是整个社区努力描述健康和疾病状态下人类蛋白质组特征的固有协作性质的一个突出范例。PPP 试点阶段称为“探索人类血浆蛋白质组”，有 55 个实验室、4 个技术委员会以及供应商和赞助商参与，其规模是国际性的。除其他成果外，PPP 生成了一个核心数据集，其中包含通过两个或更多肽段鉴定的 3020 种蛋白质，这些数据可在 EBI/PRIDE、ISB/PeptideAtlas 和密歇根大学网站上完全获取，这是进行后续分析的丰富资源。PPP 提供了广泛的注释、通过免疫测定法测量的蛋白质浓度与肽数量的相关性、用于为给定肽段匹配的多个蛋白质选择代表性蛋白质的算法，以及来自原始光谱的独立分析。PPP 的下一阶段将强调标本处理的标准化程序、用于发现和靶向蛋白质组学的强大新技术平台，以及强大的信息学工作，包括对其他生物流体的比较分析。
