Medical Molecular Biology Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Proteomics Clin Appl. 2007 Aug;1(8):780-91. doi: 10.1002/prca.200700035. Epub 2007 Jul 13.
Urinary proteomics has become one of the most attractive subdisciplines in clinical proteomics as the urine is an ideal source for the discovery of noninvasive biomarkers for kidney and nonkidney diseases. This field has been growing rapidly as indicated by >80 original research articles on urinary proteome analyses appearing since 2001, of which 28 (approximately 1/3) had been published within the year 2006. The most common technologies used in recent urinary proteome studies remain gel-based methods (1-DE, 2-DE and 2-D DIGE), whereas LC-MS/MS, SELDI-TOF MS, and CE-MS are other commonly used techniques. In addition, mass spectrometric immunoassay (MSIA) and array technology have also been applied. This review provides an extensive but concise summary of recent applications of urinary proteomics. Proteomic analyses of dialysate and ultrafiltrate fluids derived from renal replacement therapy (or artificial kidney) are also discussed.
尿蛋白质组学已成为临床蛋白质组学中最具吸引力的分支学科之一，因为尿液是发现用于诊断肾脏和非肾脏疾病的非侵入性生物标志物的理想来源。自 2001 年以来，已有 80 多篇关于尿蛋白质组分析的原始研究文章发表，表明该领域发展迅速，其中 2006 年发表的有 28 篇（约 1/3）。最近的尿蛋白质组研究中最常用的技术仍然是基于凝胶的方法（1-DE、2-DE 和 2-D DIGE），而 LC-MS/MS、SELDI-TOF MS 和 CE-MS 是其他常用的技术。此外，质谱免疫分析（MSIA）和阵列技术也已得到应用。本文综述了尿蛋白质组学的最新应用，内容广泛但简明扼要。还讨论了从肾脏替代治疗（或人工肾）中获得的透析液和超滤液的蛋白质组分析。
