Mosaiques Diagnostics and Therapeutics, Hannover, Germany.
Adv Chronic Kidney Dis. 2010 Nov;17(6):493-506. doi: 10.1053/j.ackd.2010.09.004.
Use of capillary electrophoresis coupled to mass spectrometry (CE-MS) technology in proteome analysis has increased, with a focus on the identification of biomarker peptides in clinical proteomics. Among the reported applications, the main focus is on the urinary biomarkers for kidney disease. In this review, we discuss the principal, theoretical, and practical obstacles that are encountered when using CE-MS for the analysis of body fluids for biomarker discovery. We present several examples of a successful application of CE-MS for biomarker discovery in kidney disease, implications for disease diagnosis, prognosis, and therapy evaluation, and will also discuss current challenges and possible future improvements.
毛细管电泳与质谱联用（CE-MS）技术在蛋白质组分析中的应用日益增多，尤其侧重于临床蛋白质组学中生物标志物肽的鉴定。在已报道的应用中，主要关注的是用于肾脏疾病的尿生物标志物。在本综述中，我们讨论了使用 CE-MS 分析体液进行生物标志物发现时遇到的主要、理论和实际障碍。我们还介绍了 CE-MS 在肾脏疾病生物标志物发现中的一些成功应用实例，讨论了其对疾病诊断、预后和治疗评估的影响，并探讨了当前的挑战和可能的未来改进。
