Yangtze Delta Region Institute of Tsinghua University, Jiaxing, P. R. China; Department of Dermatology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Korea.
Proteomics Clin Appl. 2008 Mar;2(3):290-300. doi: 10.1002/prca.200780063.
In this review, we summarized the recent findings regarding atopic dermatitis (AD) skin disease based on proteomic studies. AD is a chronic relapsing inflammatory skin disease typically characterized by a distribution of eczematous skin lesions with lichenification, pruritic excoriations and dry skin with wide varieties of pathophysiological aspects. We summarized the alterations of the protein expressions in the primary cultured AD cells from the patients'-biopsy samples that were mostly analyzed by 2-D PAGE and MALDI-TOF. Further, we also conducted protein-protein interaction mapping according to the obtained candidate proteins. As a result, we found that several hub proteins, i.e. heat shock 70-kDa protein 2, heat shock 70-kDa protein 9, tumor rejection antigen-1 (gp96), spermatogenesis-associated factor, protein kinase C inhibitor 1, vimentin, tenascin, semaphorin 4f (SEMA4F), complement component C1r deficiency (C1R) and apolipoprotein A (LPA), respectively, could receive important consideration in future studies. Since the mechanism of AD disease has been shown to be complex, our results may provide new clues to aid understanding of AD.
在这篇综述中，我们根据蛋白质组学研究总结了特应性皮炎（AD）皮肤病的最新发现。AD 是一种慢性复发性炎症性皮肤病，通常表现为湿疹样皮肤损伤的分布，伴有苔藓样变、瘙痒性抓痕和干燥皮肤，具有广泛的病理生理方面。我们总结了从患者活检样本中培养的原发性 AD 细胞的蛋白表达变化，这些变化主要通过 2-D PAGE 和 MALDI-TOF 进行分析。此外，我们还根据获得的候选蛋白进行了蛋白质-蛋白质相互作用作图。结果发现，一些枢纽蛋白，如热休克 70kDa 蛋白 2、热休克 70kDa 蛋白 9、肿瘤排斥抗原 1（gp96）、精子发生相关因子、蛋白激酶 C 抑制剂 1、波形蛋白、腱蛋白、神经丝蛋白 4f（SEMA4F）、补体成分 C1r 缺乏（C1R）和载脂蛋白 A（LPA），在未来的研究中可能会受到重视。由于 AD 疾病的发病机制被证明很复杂，我们的结果可能为理解 AD 提供新的线索。
